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Rabbit Anti-NOS3 Polyclonal Antibody#abs135970

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Description

Catalog-specification	Delivery time	USD price
abs135970-100ug	1-2 Weeks	301.0
abs135970-50ug	1-2 Weeks	201.0

Overview
catalog	abs135970
Description	eNOS, or endothelial nitric-oxide synthase, is an enzyme of great significance within the cardiovascular system. Its primary function is to facilitate the production of nitric oxide (NO), which plays a crucial role in regulating blood pressure, promoting vascular remodeling, and stimulating angiogenesis (1,2). To control its activity, eNOS undergoes phosphorylation at various sites. Among these sites, Ser1177 and Thr495 have been extensively studied (3). Multiple protein kinases, including Akt/PKB, PKA, and AMPK, contribute to the activation of eNOS by phosphorylating Ser1177 in response to various external signals (4,5). On the other hand, certain stimuli such as bradykinin and H2O2 promote eNOS activity by inducing phosphorylation of Ser1177 and dephosphorylation of Thr495 (6,7). Additionally, VEGF triggers the activation of eNOS through the dephosphorylation of Ser113. Notably, this dephosphorylation event can be obstructed by Cyclosporin A when stimulated by VEGF (8).
Other names	Today, I'd like to talk about cNOS. You may have heard it called by its full name, Constitutive NOS. Alternatively, you might have heard it referred to as EC-NOS, ecNOS, or Endothelial nitric oxide synthase. It's all the same thing, really! This enzyme is responsible for producing nitric oxide in our bodies. This is an important molecule that helps to regulate blood flow and blood pressure. Endothelial nitric oxide synthase is specifically found in endothelial cells, which line the inside of our blood vessels. That's why you might also hear it called Endothelial NOS or eNOS. No matter what name you use to refer to this enzyme, it's clear that it plays a vital role in maintaining cardiovascular health.
Source	Rabbit
Specificity	The NOS3 Antibody is capable of identifying total NOS3 present in the body at natural levels. The antibody has been developed to detect the endogenous levels of NOS3 and can be used for diagnostic and research purposes. By accurately identifying NOS3, this antibody can contribute to better understanding of the genetic and physiological factors that influence various diseases and conditions.
Species Reactivity	Human;Mouse;Rat
Antigen	NOS3
Application	The dilution ranges for WB, IHC, IF/ICC, and ELISA(peptide) are as follows: - WB (Western Blotting) has a dilution range of 1:500 to 1:2000. - IHC (Immunohistochemistry) has a dilution range of 1:50 to 1:200. - IF/ICC (Immunofluorescence/Immunocytochemistry) has a dilution range of 1:100 to 1:500. - ELISA (Enzyme-Linked Immunosorbent Assay) for peptide detection has a dilution range of 1:20000 to 1:40000.
Immunogen	A synthetic peptideof human NOS3.
MW	133kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Tissue specificity	Platelets, placenta, liver and kidney.
Posttranslational modification	Phosphorylation by AMPK at Ser-1177 in the presence of Ca2+-calmodulin (CaM) activates activity. In absence of Ca2+-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
Celluar localization	Cytoskeleton;Cytosol;Golgi apparatus;Nucleus;Plasma Membrane;
UniPort	P29474