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Rabbit Anti-ANG Polyclonal Antibody#abs135969

Please note that the price provided is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. We appreciate your understanding. Western blot analysis of extracts of mouse livercell lines, using ANG antibody. This product is for research use only,...

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Description

Catalog-specification	Delivery time	USD price
abs135969-100ug	1-2 Weeks	301.0
abs135969-50ug	1-2 Weeks	201.0

Please note that the price provided is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. We appreciate your understanding.

Overview
catalog	abs135969
Description	The Tie receptor ligands known as angiopoietins compose a family of proteins. Currently, four angiopoietins have been identified: angiopoietins 1, 2, 3, and 4 (Ang1, 2, 3, and 4). The interaction between Ang1 and the Tie-2 receptor triggers the receptor's autophosphorylation, activating downstream signaling pathways. This crucially contributes to the formation, maturation, and stabilization of blood vessels. Ang2, on the other hand, acts as an endothelium-specific growth factor that acts in opposition to Ang1. It promotes proinflammatory processes associated with vasculature, causing destabilization of quiescent endothelium, vascular leakage, and remodeling. In tumor tissues, Ang2 is selectively expressed and, when combined with other growth factors like VEGF, it facilitates vascular remodeling, angiogenesis, and inflammation.
Other names	Angiogenin, also known as ALS9 or Amyotrophic Lateral Sclerosis 9, is a protein that belongs to the ribonuclease RNase A family, specifically Ribonuclease 5 or RNase 5. It is encoded by the ANG gene. Angiogenin is involved in various biological processes and has been found to play a role in diseases like ALS. Angiogenin, also referred to as Ang 1 or ANG, is a human protein with an alternative name of ANGI, ANGI_HUMAN, Angiogenin ribonuclease RNase A family, 5, Epididymis luminal protein 168, HEL168, MGC22466, MGC71966, Ribonuclease RNase A Family 5, RNase 5, RNASE4, and RNASE5. ALS, or Amyotrophic Lateral Sclerosis, is a neurodegenerative disease characterized by the gradual degeneration and loss of motor neurons in the brain and spinal cord. This progressive loss of motor neurons leads to muscle weakness, paralysis, and eventually death. Angiogenin has been found to promote the growth of blood vessels, a process known as angiogenesis. It has also been implicated in tissue repair, cell survival, and the regulation of cell growth. Additionally, Angiogenin has been shown to possess ribonucleolytic activity, meaning it can degrade RNA molecules. The exact role of angiogenin in ALS is still not fully understood, but studies have suggested that dysfunction of angiogenin may contribute to the pathology of the disease. Mutations in the ANG gene have been found in a small percentage of familial ALS cases, indicating a possible genetic link. In conclusion, angiogenin, also known as ALS9 or Amyotrophic Lateral Sclerosis 9, is a protein that belongs to the ribonuclease RNase A family. It plays a role in angiogenesis, tissue repair, and cell growth regulation. Its dysfunction has been implicated in diseases like ALS.
Source	Rabbit
Specificity	The total ANG in samples can be detected using the ANG Antibody, which can recognize and bind specifically to the endogenous levels of ANG.
Species Reactivity	Human;Mouse;Rat
Antigen	ANG
Application	The dilution ratios for Western blot (WB) range from 1:500 to 1:2000. For immunohistochemistry (IHC), the dilution ratios vary from 1:50 to 1:200. When performing ELISA with peptide samples, the dilution ratios fall within the range of 1:20000 to 1:40000.
Immunogen	A synthesized peptide derived from human ANG.
MW	17kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
Tissue specificity	Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.
Celluar localization	Extracellular region or secreted;Nucleus;
UniPort	P03950