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Rabbit Anti-BIRC5 Polyclonal Antibody#abs135972

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Description

Catalog-specification	Delivery time	USD price
abs135972-50ug	1-2 Weeks	201.0
abs135972-100ug	1-2 Weeks	301.0

Overview
catalog	abs135972
Description	This particular gene belongs to the inhibitor of apoptosis (IAP) family of genes, which produce negative regulatory proteins that stop cells from undergoing apoptotic cell death. Typically, IAP family members consist of multiple baculovirus IAP repeat (BIR) domains. However, the proteins encoded by this particular gene only contain a single BIR domain, and they lack a RING finger domain at the C-terminus. While gene expression is significant during fetal development and in most cancerous tumors, levels are low in adult tissues. Various alternatively spliced transcript variants have been discovered, encoding distinct isoforms of this gene.
Other names	Survivin, also known as apoptosis inhibitor 4 or TIAP, is a protein that plays a crucial role in preventing programmed cell death, or apoptosis. It is encoded by the BIRC5 gene, also known as baculoviral IAP repeat-containing protein 5. Survivin is a member of the inhibitor of apoptosis (IAP) family of proteins, which are involved in the regulation of cell death and survival. The survivin protein is composed of 142 amino acids and has a molecular weight of approximately 16 kDa. It contains a single baculoviral IAP repeat (BIR) domain, which is characteristic of IAP family members. This domain is involved in the binding of various proteins that regulate apoptosis, including caspases, which are key executors of programmed cell death. Survivin is highly expressed in many types of cancer cells, but not in most normal tissues. This is thought to be due, in part, to the fact that survivin promotes cell proliferation and inhibits apoptosis, factors that are important for the development and progression of cancer. Because of its role in cancer cells, survivin has been studied as a potential target for cancer therapy. However, the development of effective drugs that target survivin has been challenging because of its structure and function. In addition to its role in cell survival and death, survivin has been implicated in a variety of other cellular processes, including mitosis and DNA repair. Studies have also shown that survivin is involved in the regulation of angiogenesis, the formation of new blood vessels that is necessary for the growth and spread of tumors in cancer. Overall, survivin is a multifaceted protein that plays important roles in cancer and other cellular processes. Its functions and interactions with other proteins continue to be a topic of intense research in the scientific community.
Source	Rabbit
Specificity	The detection of endogenous levels of total BIRC5 is possible with the BIRC5 Antibody. This antibody can accurately identify and measure the presence of BIRC5 within a biological sample. By targeting this specific protein, researchers can gain valuable insights into its expression and regulation in different conditions or disease states. The BIRC5 Antibody offers a reliable and convenient tool for studying the role of BIRC5 in various biological processes and may lead to a better understanding of its potential as a therapeutic target.
Species Reactivity	Human;Mouse;Rat
Antigen	BIRC5
Application	For Western blotting, the recommended dilution range is 1:500-1:2000. In the case of immunohistochemistry, a dilution range of 1:50-1:200 is advised. For immunofluorescence and immunocytochemistry, a dilution of 1:100-1:500 is recommended. When it comes to ELISA assays using a peptide substrate, the dilution range is typically 1:20000-1:40000. It is crucial to stick to these recommended dilutions to obtain accurate and reliable results.
Immunogen	A synthesized peptide derived from human BIRC5.
MW	16kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized to perform peptide affinity chromatography for the purification of the antiserum. Let me generate a highly similar content by rearranging the provided information, while ensuring that the context is preserved.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:9859993, PubMed:21364656, PubMed:20627126). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784). May play a role in neoplasia (PubMed:10626797). Inhibitor of CASP3 and CASP7 (PubMed:21536684). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797).
Tissue specificity	Expressed only in fetal kidney and liver, and to lesser extent, lung and brain (PubMed:10626797). Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas (PubMed:14741722, PubMed:16329164). Also expressed in various renal cell carcinoma cell lines (PubMed:10626797). Expressed in cochlea including the organ of Corti, the lateral wall, the interdental cells of the Limbus as well as in Schwann cells and cells of the cochlear nerve and the spiral ganglions (at protein level). Not expressed in cells of the inner and outer sulcus or the Reissner's membrane (at protein level) (PubMed:21364656, PubMed:20627126).
Posttranslational modification	Ubiquitinated by the Cul9-RING ubiquitin-protein ligase complex, leading to its degradation. Ubiquitination is required for centrosomal targeting.In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes (PubMed:14610074). Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities (PubMed:21252625). Phosphorylation at Thr-34 by CDK15 is critical for its anti-apoptotic activity (PubMed:24866247). Phosphorylation at Ser-20 by AURKC is critical for regulation of proper chromosome alignment and segregation, and possibly cytokinesis.Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation.
Celluar localization	Cytoskeleton;Cytosol;Nucleus;
UniPort	O15392