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Rabbit Anti-LTA Polyclonal Antibody#abs135973

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly reach out to our seller, Vecent. Western blot analysis of extracts of human spleen cell lines, using LTA antibody. This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs135973-100ug	1-2 Weeks	301.0
abs135973-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly reach out to our seller, Vecent.

Overview
catalog	abs135973
Description	LT-α, also called TNF-β, is a protein belonging to the TNF superfamily (1). It is expressed by various immune cells like NK cells, T cells, B cells, and lymphoid tissue-inducer cells (1). LT-α can exist as a soluble homotrimer or form membrane bound heterotrimers with lymphotoxin-β (LT α1β2 or LT α2β1), which can be cleaved from the cell surface by matrix metalloproteases (1,2). The soluble form of LT-α binds to TNFR1/TNFR2 and activates the canonical NF-κB pathway (1). On the other hand, LT α1β2 heterodimers bind to the LTβR receptor and activate the noncanonical NF-κB pathway (1). Consequently, LT-α and TNF-α exhibit similar functions. Soluble LT-α and LT α1β2 are crucial in lymphangiogenesis (3). The LT α1β2/LTβR axis plays a vital role in the development of lymphoid tissue (1,3).
Other names	Alpha lymphotoxin (LTalpha) is a member of the tumor necrosis factor (TNF) superfamily, specifically known as TNF superfamily member 1. It is also referred to as LT, LT-alpha, Lta, or lymphotoxin-alpha. TNF superfamily member 1, also known as TNF B or TNF-beta, is another name for LTalpha. TNF, lymphocyte-derived, TNFB, TNFB_HUMAN, TNFbeta, TNFSF1, TNLG1E, and tumor necrosis factor ligand superfamily member 1E are all aliases for LTalpha. This protein, known as tumor necrosis factor ligand 1E, plays a significant role in immune responses and inflammation. Thus, various terms exist to describe LTalpha due to its importance in these biological processes.
Source	Rabbit
Specificity	The total LTA levels in the body can be detected using the LTA Antibody, which specifically targets endogenous levels of LTA.
Species Reactivity	Human;Mouse;Rat
Antigen	LTA
Application	Western blot (WB) is typically performed using dilutions ranging from 1:500 to 1:2000. Similarly, for immunohistochemistry (IHC), the recommended dilutions are between 1:50 and 1:200. On the other hand, ELISA assays using peptide antigens usually require higher dilutions, ranging from 1:20000 to 1:40000. These dilution ranges ensure optimal signal detection and accurate quantification of target proteins or antigens in the respective assays.
Immunogen	A synthesized peptide derived from human LTA.
MW	22kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	P01374