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Rabbit Anti-TIMP1 Polyclonal Antibody#abs131990

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Description

Catalog-specification	Delivery time	USD price
abs131990-100ug	1-2 Weeks	301.0
abs131990-50ug	1-2 Weeks	201.0

Overview
catalog	abs131990
Description	Metalloproteinase inhibitors like TIMP1 form complexes with enzymes such as collagenases, effectively rendering them inactive by binding to their essential zinc cofactor in a way that cannot be reversed. In addition to this inhibitory role, TIMP1 has been found to have a role in erythropoiesis when studied in laboratory settings. Unlike IL-3, TIMP1's effects on erythroid progenitors are specific to humans and mice only, stimulating their growth and promoting differentiation. It is worth noting that TIMP1 acts on various metalloproteinases including MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13, and MMP-16.
Other names	One of the key players in regulating the activity of matrix metalloproteinases (MMPs) is the tissue inhibitor of metalloproteinases (TIMPs). TIMP-1, also known as collagenase inhibitor or fibroblast collagenase inhibitor, is a human protein that belongs to the TIMP family. TIMP-1 is a potent and specific inhibitor of MMPs, particularly MMP-1 and MMP-9. It also has erythroid-potentiating activity (EPA) and is involved in regulating erythropoiesis. Other names that TIMP-1 goes by include metalloproteinase inhibitor 1, metalloproteinase inhibitor 1 precursor, and FLJ90373. TIMP-1 has been identified as a potential diagnostic and prognostic biomarker for various diseases, including cancer, cardiovascular diseases, and neurological disorders.
Source	Rabbit
Specificity	The endogenous levels of total TIMP1 can be effectively detected with the TIMP1 Antibody. To provide similar content by rearranging the information, you can say: The TIMP1 Antibody is capable of detecting the total TIMP1 levels naturally present in an organism. This antibody is highly effective in determining the endogenous levels of TIMP1.
Species Reactivity	Human;Mouse;Rat
Antigen	TIMP1
Application	To achieve optimal results in Western blotting, dilutions of 1:500 to 1:2000 are recommended. When performing immunofluorescence or immunocytochemistry, dilutions of 1:100 to 1:500 are appropriate. For ELISA with a peptide substrate, recommended dilutions are between 1:20000 and 1:40000. It is important to closely follow these suggested dilution protocols in order to obtain reliable and accurate data for your experiments.
Immunogen	A synthesized peptide derived from human TIMP1.
MW	24kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.
Tissue specificity	Detected in rheumatoid synovial fluid (at protein level).
Posttranslational modification	The activity of TIMP1 is dependent on the presence of disulfide bonds.N-glycosylated.
Celluar localization	Endoplasmic reticulum;Extracellular region or secreted;
UniPort	P01033