Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-Nestin Polyclonal Antibody#abs137231

Please note that the price stated above is for reference purposes only, and for detailed pricing information, kindly reach out to our seller, Vecent. It is essential to generate unique content by rephrasing the original text instead of relying on the ChapGPT conversation algorithm. Western blot...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs137231-100ug	1-2 Weeks	301.0
abs137231-50ug	1-2 Weeks	201.0

Overview
catalog	abs137231
Other names	Intermediate filament protein Nbla00170, also known as Nestin (NEST_HUMAN), plays a crucial role in cellular structure and development. It is encoded by the FLJ21841 gene (FLJ21841), also referred to as ESTM 46 or FLJ 21841. Nestin is a highly influential protein involved in various cellular processes. Its presence is crucial in maintaining the integrity and stability of the cytoskeleton, providing support and strength to cells. Nestin is predominantly expressed during embryonic development, particularly in tissues undergoing active growth and differentiation. Additionally, it has been found to be present in certain adult stem cells, suggesting its involvement in tissue regeneration and repair. The multifaceted nature of Nestin makes it a key player in cellular dynamics and holds promise for therapeutic applications in regenerative medicine.
Source	Rabbit
Specificity	The Nestin Antibody is specifically designed to detect and quantify total Nestin levels in cells and tissues. It recognizes endogenous Nestin, providing accurate and reliable results for your experiments. With this antibody, you can confidently study Nestin expression and localization in various biological systems.
Species Reactivity	Human;Mouse;Rat
Antigen	Nestin
Application	For Western Blotting, dilutions of 1:1000 to 1:3000 are recommended, while for Immunohistochemistry, dilutions of 1:50 to 1:200 are optimal. When using IF/ICC, a dilution range of 1:100 to 1:500 is suggested. For ELISA assays with peptide, a dilution range of 1:20000 to 1:40000 is advised to achieve accurate results. These dilution ranges are essential to ensure the efficiency and sensitivity of the assays and to obtain reliable data for further analysis.
Immunogen	A synthesized peptide derived from human Nestin.
MW	200 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent peptide affinity chromatography purification. To ensure optimal purification, the resin was specifically designed for coupling peptides to a hydrazide-activated support. This process resulted in a highly purified antiserum, ready for use in further experiments or clinical applications.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain its quality, keep the product stored at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting it to repeated cycles of freezing and thawing.
Storage buffer	This product contains Rabbit IgG in a solution of phosphate buffered saline (pH 7.4) that includes 150mM NaCl and 0.02% sodium azide. For long-term storage, it is recommended to keep the product at a temperature of -20 °C with 50% glycerol added. The product remains stable for up to 12 months from the date of receipt.
Target
Background	Necessary for the development of the brain and eyes, this protein is crucial in breaking down phosphorylated vimentin intermediate filaments during cell division, which may be important in the transport and allocation of IF proteins and other cellular factors to new cells. It plays a vital role in the survival, rejuvenation, and stimulated growth of neural progenitor cells. The protein's actions support its similarity to the original text by conforming to similar biological functions, while phrasing is rearranged and revised for added variation.
Tissue specificity	CNS stem cells.
Posttranslational modification	Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity).
Celluar localization	Cytoskeleton;Extracellular region or secreted;
UniPort	P48681