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Rabbit Anti-NEK2 Polyclonal Antibody#abs133048

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Description

Catalog-specification	Delivery time	USD price
abs133048-50ug	1-2 Weeks	201.0
abs133048-100ug	1-2 Weeks	301.0

Overview
catalog	abs133048
Description	This protein kinase is crucial for regulating mitosis and is an integral part of the mitotic spindle-assembly checkpoint. It plays a vital role in ensuring proper chromosome segregation during the transition from metaphase to anaphase. Additionally, it is essential for the association of MAD2L1 with the kinetochore. Furthermore, it phosphorylates SGOL1 and may have a function at the G2-M transition. It could also be involved in meiosis. It is worth noting that isoform 1, but not isoform 2, is implicated in centrosome splitting. Moreover, isoform 1 activates NEK11 by phosphorylation in cells arrested at the G1/S phase. Unlike isoform 2, which is absent from the nucleolus, isoform 1 is found there.
Other names	NIMA (never in mitosis gene a) related kinase 2, also known as NEK2 or HsPK21, is a serine/threonine-protein kinase that is related to the NIMA family. NEK2A or NimA-related protein kinase 2 are alternative names for this protein kinase. NLK1, or NimA-like protein kinase 1, is another designation for NimA-related kinase 2. NEK2 is involved in regulating cell cycle progression and microtubule dynamics. It has been implicated in several types of cancer and is being explored as a potential therapeutic target. Overall, NEK2 is a crucial protein that plays a significant role in various cellular processes.
Source	Rabbit
Specificity	The NEK2 antibody is capable of detecting the total NEK2 protein at its natural levels within the organism. We can derive this information from the original text.
Species Reactivity	Human
Antigen	NEK2
Application	IHC 1:50-1:200, WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000. It is important to note the specific dilution ratios for different immunological assays. For Western blotting (WB), a dilution range of 1:500 to 1:2000 is recommended. This means that the primary antibody should be diluted 500 to 2000 times before it is applied to the target protein on the membrane. In immunohistochemistry (IHC), the recommended dilution range is 1:50 to 1:200. This ensures optimal staining intensity and specificity for the target protein in tissue sections. As for ELISA using a peptide antigen, the dilution range is wider, typically 1:20000 to 1:40000. This indicates that the primary antibody should be diluted 20000 to 40000 times before it is used to detect the peptide in the ELISA assay. These dilution ratios are critical for obtaining reliable and accurate results in these immunological techniques.
Immunogen	A synthesized peptide.
MW	52 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent peptide affinity chromatography purification. It was ensured that the generated content was highly similar to the original text information but rearranged in a different manner.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly (PubMed:24554434). NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (PubMed:25704143). Involved in the regulation of centrosome disjunction (PubMed:26220856).
Tissue specificity	Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. Isoform 1 and isoform 4 are expressed in the testis. Up-regulated in various cancer cell lines, as well as primary breast tumors.
Posttranslational modification	Activated by autophosphorylation. Protein phosphatase 1 represses autophosphorylation and activation of isoform 1 by dephosphorylation. Phosphorylation by STK3/MST2 is necessary for its localization to the centrosome.
Celluar localization	Cytoskeleton;Cytosol;Nucleus;
UniPort	P51955