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Rabbit Anti-Cdc16 Polyclonal Antibody#abs140393

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Description

Catalog-specification	Delivery time	USD price
abs140393-100ug	1-2 Weeks	301.0
abs140393-50ug	1-2 Weeks	201.0

Overview
catalog	abs140393
Other names	APC6, also known as Anaphase-promoting complex subunit 6 or Anaphase promoting complex subunit 6, plays a crucial role in cell division. It is the human homolog of CDC16, a protein involved in cell division cycle 16 in Saccharomyces cerevisiae. APC6, also referred to as CDC16_HUMAN or CDC16Hs, is a component of the anaphase-promoting complex (APC), an enzyme complex that controls the progression of the cell cycle. APC6, encoded by the gene ANAPC6, is essential for the proper execution of mitosis, the process by which a cell divides into two daughter cells. It functions by targeting specific proteins for degradation, thereby ensuring accurate chromosome segregation and cell division. In the absence of APC6, errors in chromosome segregation may occur, leading to cell cycle defects and genomic instability. CUT9, another name for APC6, is an important player in the regulation of cell division. It forms a complex with other subunits of the APC, collectively referred to as the cyclosome, to carry out its functions. The cyclosome functions as an E3 ubiquitin ligase, marking specific proteins for destruction by the proteasome. By targeting key cell cycle regulators for degradation, APC6 helps to ensure the timely progression through mitosis and prevent the accumulation of abnormal cells. The dysregulation of APC6 has been implicated in various diseases, including cancer. Mutations in ANAPC6 or alterations in APC6 expression levels can disrupt the precise control of cell division, leading to uncontrolled cell growth and tumor formation. Therefore, APC6 represents a potential therapeutic target for the development of novel anti-cancer strategies. In conclusion, APC6, also known as Anaphase promoting complex subunit 6, is a critical component of the cell division machinery. Its role in targeting specific proteins for degradation ensures the faithful execution of mitosis. Dysregulation of APC6 can have severe consequences, highlighting the importance of understanding its function and regulation in both normal development and disease.
Source	Rabbit
Specificity	The levels of total Cdc16, both exogenous and endogenous, can be accurately detected using the Cdc16 Antibody. It is a highly reliable tool that specifically targets and identifies the presence of Cdc16 in biological samples. This antibody-based assay provides valuable insights into the quantitative assessment of Cdc16 levels in various experimental settings. By detecting the endogenous levels of Cdc16, researchers can gain a comprehensive understanding of its role and function in cellular processes.
Species Reactivity	Human
Predictive reaction species	Zebrafish;Chicken;Rabbit;Xenopus;Dog;Horse;Bovine;Sheep;Pig
Antigen	Cdc16
Application	The recommended dilutions for Western Blot (WB) are 1:1000, for Immunofluorescence (IF) and Immunocytochemistry (ICC) are 1:100-1:500, and for ELISA (peptide) are 1:20000-1:40000. Let's create a similar content by reorganizing the provided information.
Immunogen	A synthesized peptide derived from human Cdc16.
MW	72kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized for the peptide affinity chromatography purification of the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting it to multiple freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Posttranslational modification	Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.
Celluar localization	Cytoskeleton;Cytosol;Nucleus;
UniPort	Q13042