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Phospho-AMPKB1 (Ser108) Rabbit Polyclonal Antibody#abs140067

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Description

Catalog-specification	Delivery time	USD price
abs140067-100ug	1-2 Weeks	301.0
abs140067-50ug	1-2 Weeks	201.0

Overview
catalog	abs140067
Other names	The protein 1300015D22Rik, also known as AMP-activated protein kinase beta-1 subunit or AAKB1_HUMAN, is a non-catalytic subunit of AMP-activated protein kinase. It is involved in regulating energy homeostasis in cells by sensing changes in the AMP/ATP ratio. This protein plays a key role in many cellular processes, including glucose and lipid metabolism, protein synthesis, and autophagy. It is encoded by the PRKAB1 gene and has various aliases, such as AMPK-BETA-1, AMPKb, and HAMPKb. Other aliases include AU021155, E430008F22, and MGC17785. The protein is widely expressed in different tissues, including the liver, muscle, and adipose tissue. Its dysregulation has been linked to various metabolic disorders, such as obesity, type 2 diabetes, and cancer. Hence, this protein represents an attractive target for drug discovery and therapeutic interventions for these diseases.
Source	Rabbit
Specificity	The AMPKB1 (Phospho-Ser108) Antibody specifically detects the presence of AMPKB1 that has been phosphorylated at Ser108. This antibody has been designed to detect endogenous levels of this protein only when it is in a phosphorylated state at this particular site.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Bovine;Horse;
Antigen	AMPKB1
Application	The recommended dilutions for Western Blot (WB) range from 1:1000 to 1:3000. For Immunohistochemistry (IHC), the suggested dilutions are between 1:50 and 1:200. In the case of Immunofluorescence/Immunocytochemistry (IF/ICC), dilutions of 1:100 to 1:500 are commonly used. For ELISA (peptide), the ideal dilutions fall within the range of 1:20000 to 1:40000. It is important to adhere to these dilution guidelines to ensure accurate and reliable results.
Immunogen	One possible way to generate highly similar content by rearranging the above text could be: The peptide synthesized was derived from human AMPKB1 specifically targeting Phospho-Ser108. This implies that the peptide mimics the phosphorylation of this amino acid residue which is crucial for regulating the function of the AMP-activated protein kinase (AMPK). By synthesizing this peptide, researchers can investigate the role of Phospho-Ser108 in various cellular processes and potentially develop drugs that target this pathway. AMPK is known to be involved in energy homeostasis, metabolism, and autophagy, making it a promising target for anti-cancer therapies and treatment of metabolic disorders. Therefore, the synthesis of this novel peptide opens up new avenues for understanding the complex cellular signaling pathways regulated by AMPK and potential therapeutic interventions based on this knowledge.
MW	38kDa
Properties
Concentration	1mg/ml
purification	The purified rabbit serum was used to generate the antibody through a process of affinity purification. This was achieved by passing the serum through both phospho- and non-phospho-peptide affinity columns sequentially, resulting in a highly pure and specific antibody.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Posttranslational modification	Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
Celluar localization	Cytosol;Nucleus;
UniPort	Q9Y478