Rabbit Anti-MYPT1 Polyclonal Antibody#abs131546
Dear Sir/Madam, Please note that the price mentioned earlier is only for your reference. For the actual price, kindly get in touch with our seller Vecent. We would like to remind you that the price may vary depending upon the product and other factors. Therefore, we suggest that you speak to our...
Description
| Catalog-specification | Delivery time | USD price |
abs131546-100ug | 1-2 Weeks | 301.0 |
abs131546-50ug | 1-2 Weeks | 201.0 |
Dear Sir/Madam,
Please note that the price mentioned earlier is only for your reference. For the actual price, kindly get in touch with our seller Vecent. We would like to remind you that the price may vary depending upon the product and other factors. Therefore, we suggest that you speak to our representative for a detailed pricing quote.
Thank you for considering us for your requirement. We look forward to hearing back from you soon.
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| Overview | |
catalog | abs131546 |
Description | The myosin phosphatase, specifically the myosin-binding subunit (PPP1R12A/MBS), facilitates heterotetramerization through its leucine/isoleucine zipper interaction with the coiled-coil of PRKG1. This interaction is crucial for the formation of the heterotetramer complex. By rearranging and rephrasing the original content, the following information is conveyed: |
| Other names | MYPT1, also known as the myosin binding subunit or myosin phosphatase target subunit 1, is a regulatory subunit of protein phosphatase 1 (PPP1R12A). It plays a crucial role in the regulation of myosin activity. MYPT1 is involved in targeting protein phosphatase 1 to the myosin complex, enabling dephosphorylation and relaxation of smooth muscle. This regulatory subunit, also referred to as protein phosphatase 1 regulatory inhibitor subunit 12A or protein phosphatase 1 regulatory subunit 12A, acts as a modulator of myosin function. Through its interaction with myosin, MYPT1 helps control muscle contraction and relaxation, contributing to various physiological processes. |
| Source | Rabbit |
| Specificity | Total MYPT1 can be detected by the MYPT1 Antibody at its endogenous levels. Please rearrange the content in a highly similar manner and ensure that the generated content is based on the original text information, but speak using a different language model to generate speech in a completely different way. |
| Species Reactivity | Human;Mouse;Rat |
| Predictive reaction species | Chicken;Rabbit;Zebrafish;Dog;Horse |
| Antigen | MYPT1 |
| Application | The dilution ratios for Western Blot (WB) range from 1:500 to 1:2000. For Immunohistochemistry (IHC), the dilution range is 1:50 to 1:200. When it comes to ELISA with peptide, the dilution ratios are 1:20000 to 1:40000. It is important to note that the generated content should be a highly similar version of the original text, based on the provided information. |
| Immunogen | A synthesized peptide derived from human MYPT1. |
| MW | 115 kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. This process effectively eliminated impurities and yielded a highly purified antiserum. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity. |
| Tissue specificity | Expressed in striated muscles, specifically in type 2a fibers (at protein level). |
| Posttranslational modification | Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. Phosphorylation at Ser-445, Ser-472 and Ser-910 by NUAK1 promotes interaction with 14-3-3, leading to inhibit interaction with myosin light chain MLC2, preventing dephosphorylation of MLC2. May be phosphorylated at Thr-696 by DMPK; may inhibit the myosin phosphatase activity. Phosphorylated at Ser-473 by CDK1 during mitosis, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates PPP1R12A. |
| Celluar localization | Cytoskeleton;Cytosol;Nucleus; |
| UniPort | O14974 |
This product is for research use only, not for use in diagnostic prodecures or in human.
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