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Rabbit Anti-MAP3K14 Polyclonal Antibody#abs132730

Description

Catalog-specification	Delivery time	USD price
abs132730-100ug	1-2 Weeks	301.0
abs132730-50ug	1-2 Weeks	201.0

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Overview
catalog	abs132730
Description	Lymphotoxin beta-activated kinase is exclusively responsible for activating NF-kappa-B and enhancing its transcriptional activity. It plays a crucial role in initiating the non-canonical pathway of NF-kappa-B activation by promoting the proteolytic processing of NFKB2/P100. Interestingly, this kinase may exhibit selective binding to specific receptors, suggesting its involvement in receptor-specific signaling mechanisms.
Other names	MAP3K14/NIK（MITOGEN14NF-β），。，NF-κB（-κB）。NF-κB，，、。MAP3K14，MAP2K3MAP2K6，NF-κB。，。MAP3K14，。
Source	Rabbit
Specificity	The MAP3K14 antibody is capable of detecting the total amount of endogenous MAP3K14 present in a sample. This means that it can identify and measure the protein levels of MAP3K14 that are already existing in a biological system.
Species Reactivity	Human;Mouse
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Bovine;Horse;
Antigen	MAP3K14
Application	The recommended dilutions for Western blotting range from 1:500 to 1:2000, while for immunohistochemistry, a dilution of 1:50 to 1:200 is suggested. For ELISA analysis using peptides, dilutions of 1:20000 to 1:40000 are appropriate. To obtain accurate and reliable results, it is essential to carefully determine the proper dilution for each experiment. Dilution factors that are too high or too low can lead to false-negative or false-positive results, respectively. It is recommended to perform a titration experiment to determine the optimal dilution for each individual application.
Immunogen	A synthesized peptide.
MW	105.4 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. To obtain a highly pure yield, this technique was employed.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
Tissue specificity	Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
Posttranslational modification	Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.
Celluar localization	Cytosol;Nucleus;
UniPort	Q99558