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Rabbit Anti-IL1R2 Polyclonal Antibody#abs136202

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Description

Catalog-specification	Delivery time	USD price
abs136202-100ug	1-2 Weeks	301.0
abs136202-50ug	1-2 Weeks	201.0

Overview
catalog	abs136202
Description	The interleukin 1 receptor family consists of a cytokine receptor called the protein encoded by this gene. It selectively binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA) to act as a decoy receptor that prevents the activity of its ligands. Interleukin 4 (IL4) helps to counteract the effects of interleukin 1 by facilitating the expression and release of this cytokine. This gene is located on chromosome 2q12 and forms a cytokine receptor gene cluster with three other genes. Alternative splicing events result in various protein isoforms and multiple transcript variants, including both membrane-bound and soluble proteins. Proteolytic cleavage produces a soluble protein as well.
Other names	The IL1 receptor II, also known as CDw121b or CD121, is a member of the CD121 antigen-like family. It is also referred to as CD121B antigen or IL-1R-beta. Other designations for this protein include IL1R-2, IL-1R2, IL-1RT-2, IL-1RT2, Il1r-2, and IL1RB. Additionally, it is known as Interleukin 1 receptor type II variant 3, Interleukin 1 receptor, beta, type II, Interleukin 1 receptor, type II, Interleukin-1 receptor beta, and Type II interleukin-1 receptor, beta. This protein is encoded by the IL1R2 gene and is widely expressed in many tissue types. It acts as a decoy receptor for the pro-inflammatory cytokine IL-1, ultimately inhibiting its activity. Overall, the IL1 receptor II plays an important role in regulating the immune response and inflammatory processes in the body.
Source	Rabbit
Specificity	Total IL1R2 can be identified using IL1R2 Antibody, which is capable of detecting its endogenous levels.
Species Reactivity	Human;Mouse
Antigen	IL1R2
Application	The recommended dilution for Western blotting is 1:500-1:2000, for Immunohistochemistry it's 1:50-1:200, and for ELISA using peptide, it's 1:20000-1:40000. These dilutions have been suggested to ensure efficient detection of the target protein. It is important to follow these guidelines to ensure that the results obtained are accurate and reliable. Western blotting, Immunohistochemistry, and ELISA are commonly used methods in molecular biology to detect and quantify specific proteins in biological samples. These techniques are widely used in research and clinical applications to study the expression and localization of proteins in different tissues and cells. With accurate results from these assays, valuable insights can be gained into the role of proteins in various biological processes.
Immunogen	A synthesized peptide derived from human IL1R2.
MW	45kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLinkâ„¢ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 Â°C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 Â°C.Stable for 12 months from date of receipt.
Target
Background	Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
Posttranslational modification	A soluble form (sIL1R2) can also be produced by proteolytic cleavage at the cell surface (shedding) involving a metalloproteinase; hovever, several sIL1R2 forms ranging from 45 and 60 kDa are reported.
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	P27930