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Rabbit Anti-IL1F9 Polyclonal Antibody#abs139633

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Description

Catalog-specification	Delivery time	USD price
abs139633-100ug	1-2 Weeks	301.0
abs139633-50ug	1-2 Weeks	201.0

Overview
catalog	abs139633
Other names	IL 1 epsilon, also known as IL 1F9, IL 1H1, or IL 1RP2, is a member of the interleukin-1 family. It has been identified as a mediator of inflammation and is involved in various inflammatory processes. IL 1 epsilon is highly related to IL 1 related protein 2, or IL 1RP2, and shares many similar characteristics with this protein. IL 1 epsilon is also referred to as IL-1 epsilon or Interleukin-1 epsilon. It is a cytokine that plays a key role in the regulation of immune and inflammatory responses. IL 1 epsilon is produced by a variety of cells, including macrophages, epithelial cells, and fibroblasts. IL 1 epsilon is also commonly known as Interleukin 1 family member 9 and Interleukin 1 homolog 1. It has been shown to be involved in the regulation of various cellular processes, including cell growth, differentiation, and apoptosis. IL 1 epsilon is highly related to IL 36 gamma, or IL36G, which is another member of the interleukin-1 family. IL 1 epsilon, IL 1F9, IL 1H1, and IL 1RP2 are all different names used to describe this important cytokine. Despite the different names, all of these terms refer to the same protein and are used interchangeably in the scientific literature.
Source	Rabbit
Specificity	The IL1F9 Antibody is capable of detecting the total levels of endogenous IL1F9. This means that it can effectively pinpoint the presence of IL1F9 in a sample and provide accurate results. The antibody works by binding to the IL1F9 protein and can be used to study the protein's behavior in various biological processes. Overall, the IL1F9 Antibody is a valuable tool for researchers looking to investigate the role of IL1F9 in different cellular pathways or diseases.
Species Reactivity	Human;Mouse
Antigen	IL1F9
Application	For Western blotting, a dilution ratio of 1:1000-3000 is typically used. Meanwhile, in immunohistochemistry studies, a dilution of 1:200 is typically optimal. When utilizing ELISA assays using peptides, a recommended dilution range is between 1:20000-1:40000. Keep in mind that these ratios may vary depending on the specific experimental conditions and requirements. Overall, these dilutions should be used to ensure accuracy and reliability in detecting proteins and peptides in various types of experiments and analyses.
Immunogen	A synthesized peptide derived from human IL1F9.
MW	19 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. The resulting product was highly purified and ensured the removal of any impurities present. This approach allowed for the isolation of specific peptides and the generation of a highly concentrated antiserum that can be further used for various downstream applications. Overall, the SulfoLink™ Coupling Resin based peptide affinity chromatography proved to be effective in purifying the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure maximum shelf life, it is recommended to store the product at a temperature of -20 °C for up to one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles as this can potentially compromise its efficacy. Therefore, it is advised to handle the product with care to prevent any unnecessary temperature fluctuations. Following these storage guidelines will help maintain the integrity and quality of the product over time.
Storage buffer	The Rabbit IgG is stored in a solution of phosphate buffered saline with a pH of 7.4, containing 150mM of NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store it at -20 °C, where it remains stable for a period of 12 months from the date of receipt. This ensures the preservation of its quality and effectiveness for future use.
Target
Background	Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1; also stimulates its own expression and that of the prototypic cutaneous proinflammatory parameters TNF-alpha, S100A7/psoriasin and inducible NOS. May play a role in proinflammatory responses during particular neutrophilic airway inflammation: activates mitogen-activated protein kinases and NF-kappa B in primary lung fibroblasts, and stimulates the expression of IL-8 and CXCL3 and Th17 chemokine CCL20 in lung fibroblasts. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus.
Tissue specificity	Highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. Expressed in bronchial epithelial. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional psoriasis skin. Expressed in monocyte-derived dendritic cells and M1 macrophages.
Posttranslational modification	N-terminal truncation leads to a dramatic enhancement of its activity (>1000-fold).
Celluar localization	Extracellular region or secreted;
UniPort	Q9NZH8