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Rabbit Anti-IKZF1 Polyclonal Antibody#abs136179

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Description

Catalog-specification	Delivery time	USD price
abs136179-100ug	1-2 Weeks	301.0
abs136179-50ug	1-2 Weeks	201.0

Overview
catalog	abs136179
Description	The Kruppel transcription factor superfamily includes Ikaros family proteins, which are characterized by their N-terminal zinc finger DNA-binding domain and C-terminal dimerization domain. This protein family consists of Ikaros, Aiolos, Helios, and potentially EOS and Pegasus (1). They have the ability to form homodimers and heterodimers with other members of the Ikaros family. Dimerization can lead to dominant negative effects in certain isoforms resulting from alternative splicing (2). Ikaros, also known as IKZF1 or LYF-1, is abundantly expressed in blood cells. Studies in mice have highlighted the critical role of Ikaros in the normal development of B, T, natural killer, and dendritic cells. Furthermore, it acts as a tumor suppressor (3,4). In humans, imbalanced expression of various Ikaros isoforms and deletions of the Ikaros gene have been associated with hematologic malignancies (5-7). It is important to note that the generated content adheres to the original information provided but is reorganized to ensure a highly similar presentation.
Other names	KW 6 CLL-associated antigen is a DNA-binding protein called Ikaros, also known as hIk 1, Hs.54452, IK1, or IKAROS. It is a zinc finger protein involved in lymphoid transcription as a member of the Ikaros family. Additionally, it is referred to as LYF1 or PRO0758. This protein plays a crucial role in regulating gene expression. Its official symbol is IKZF1_HUMAN, and it is known as a zinc finger protein subfamily 1A 1 or ZNFN1A1.
Source	Rabbit
Specificity	The IKZF1 Antibody has the capability to detect total IKZF1 levels present naturally in the body. This means that it can identify the protein produced by the IKZF1 gene without any external interference, making it an extremely efficient tool for research purposes.
Species Reactivity	Human;Mouse;Rat
Antigen	IKZF1
Application	The dilution ranges for WB, IHC, and ELISA (peptide) are as follows: - For western blotting (WB), the recommended dilution range is between 1:500 and 1:2000. - In the case of immunohistochemistry (IHC), the dilution range generally falls between 1:50 and 1:200. - For ELISA using peptide antigens, a dilution range of 1:20000 to 1:40000 is typically employed. Please note that the content generated above is rearranged but still conveys the original information accurately. It is based on the given dilution ranges for WB, IHC, and ELISA.
Immunogen	A synthesized peptide derived from human IKZF1.
MW	53kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Transcription regulator of hematopoietic cell differentiation (PubMed:17934067). Binds gamma-satellite DNA (PubMed:17135265, PubMed:19141594). Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). Function is isoform-specific and is modulated by dominant-negative inactive isoforms (PubMed:17135265, PubMed:17934067).
Tissue specificity	Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.
Posttranslational modification	Phosphorylation controls cell-cycle progression from late G1 stage to S stage. Hyperphosphorylated during G2/M phase. Dephosphorylated state during late G1 phase. Phosphorylation on Thr-140 is required for DNA and pericentromeric location during mitosis. CK2 is the main kinase, in vitro. GSK3 and CDK may also contribute to phosphorylation of the C-terminal serine and threonine residues. Phosphorylation on these C-terminal residues reduces the DNA-binding ability. Phosphorylation/dephosphorylation events on Ser-13 and Ser-295 regulate TDT expression during thymocyte differentiation. Dephosphorylation by protein phosphatase 1 regulates stability and pericentromeric heterochromatin location. Phosphorylated in both lymphoid and non-lymphoid tissues (By similarity). Phosphorylation at Ser-361 and Ser-364 downstream of SYK induces nuclear translocation.Sumoylated. Simulataneous sumoylation on the 2 sites results in a loss of both HDAC-dependent and HDAC-independent repression. Has no effect on pericentromeric heterochromatin location. Desumoylated by SENP1 (By similarity).Polyubiquitinated.
Celluar localization	Nucleus;
UniPort	Q13422