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Rabbit Anti-AMBP Polyclonal Antibody#abs136176

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Description

Catalog-specification	Delivery time	USD price
abs136176-100ug	1-2 Weeks	301.0
abs136176-50ug	1-2 Weeks	201.0

Overview
catalog	abs136176
Description	The gene under discussion codes for a complex glycoprotein that is secreted into the blood plasma. After undergoing proteolytic processing, it gives rise to two distinct functioning proteins: alpha-1-microglobulin and bikunin. Alpha-1-microglobulin belongs to the lipocalin transport protein superfamily and is believed to regulate inflammatory processes. On the other hand, bikunin falls under the Kunitz-type protease inhibitor superfamily and plays a crucial role in many physiological and pathological processes. This gene is situated on chromosome 9 alongside other lipocalin genes.
Other names	Alpha-1 microglobulin, also known as AMBP or bikunin, is a precursor for the protein bikunin. It is a glycoprotein that is heterogeneous in charge and forms complexes. It is also referred to as complex-forming glycoprotein. Alpha-1 microglobulin has several other names such as growth inhibiting protein 19, uronic-acid-rich protein, tryptatin, and uristatin. It is a potent inhibitor of proteolytic enzymes and exhibits anti-inflammatory properties. It is also believed to play a role in the regulation of the complement system. Alpha-1 microglobulin is found in high concentrations in urine and is used as a biomarker for kidney function. It is frequently used in clinical laboratories to help diagnose kidney diseases.
Source	Rabbit
Specificity	The AMBP Antibody is capable of detecting total levels of endogenous AMBP.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Dog;
Antigen	AMBP
Application	Here are some variations of the original text: - For Western blotting, dilute the primary antibody at a range of 1:500 to 1:2000. For immunohistochemistry, the primary antibody should be diluted at 1:50 to 1:200. If you're performing ELISA using a peptide antigen, the recommended dilution is between 1:20000 to 1:40000. - Depending on the application, the primary antibody should be diluted accordingly. Western blotting requires a dilution range of 1:500 to 1:2000, whereas immunohistochemistry requires a dilution of 1:50 to 1:200. For ELISA assays using peptides as antigens, a dilution range of 1:20000 to 1:40000 is recommended. - If you're performing Western blotting, dilute the primary antibody between 1:500 to 1:2000. For immunohistochemistry assays, the dilution range should be between 1:50 to 1:200. For ELISA assays that use peptide antigens, a recommended dilution range of 1:20000 to 1:40000 has been suggested.
Immunogen	A synthesized peptide derived from human AMBP.
MW	39kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
Tissue specificity	Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
Posttranslational modification	The precursor is proteolytically processed into separately functioning proteins.3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.N- and O-glycosylated. N-glycan heterogeneity at Asn-115: Hex5HexNAc4 (major), Hex6HexNAc5 (minor) and dHex1Hex6HexNAc5 (minor). N-glycan at Asn-250: Hex5HexNAc4. O-linkage of the glycosaminoglycan, chondroitin sulfate, at Ser-215 allows cross-linking between the three polypeptide chains.
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	P02760