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Rabbit Anti-IKK-beta Polyclonal Antibody#abs131612

Please note that the price mentioned above is only for reference. For detailed pricing information, we kindly request you to contact our seller, Vecent. Western blot analysis of IKK-beta expression in LPS treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific...

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Description

Catalog-specification	Delivery time	USD price
abs131612-50ug	1-2 Weeks	201.0
abs131612-100ug	1-2 Weeks	301.0

Please note that the price mentioned above is only for reference. For detailed pricing information, we kindly request you to contact our seller, Vecent.

Overview
catalog	abs131612
Description	IKK-beta, a member of the IKK family, acts as a kinase. It plays a crucial role in phosphorylating inhibitors of NF-kappa-B, leading to the dissociation of the inhibitor/NF-kappa-B complex. Consequently, the inhibitor undergoes degradation. IKK-beta primarily exists as a heterodimer with IKK-alpha, but it can also form a homodimer.
Other names	The protein known as I kappa B kinase beta is also referred to as IKK beta, IKK-B, IKK-beta, IKK2, IKKB, IKKB_HUMAN, IMD15, Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta, Inhibitor of nuclear factor kappa-B kinase subunit beta, NFKBIKB, and Nuclear factor NF-kappa-B inhibitor kinase beta. These terms all describe the same protein that plays a crucial role in the activation of the transcription factor, nuclear factor kappa B (NF-kB). This protein complex is important in the regulation of immune and inflammatory responses, as well as in the control of cell growth and survival. Understanding the function and regulation of IKK beta is key to developing treatments for a variety of diseases including chronic inflammation, autoimmune disorders, and cancer.
Source	Rabbit
Specificity	The total level of endogenous IKK-beta can be detected by using IKK-beta Antibody. This particular antibody is highly effective in detecting the presence of IKK-beta in a sample. It is capable of providing accurate results, which can be used to determine the level of IKK-beta in different types of cells or tissues. The IKK-beta Antibody is a reliable tool for scientists and researchers who are interested in studying the functions and mechanisms of IKK-beta in various biological systems.
Species Reactivity	Human;Mouse;Rat;Monkey
Predictive reaction species	Xenopus;Zebrafish;Rabbit;Pig;Dog;Horse;Bovine
Antigen	IKK-β
Application	For Western blotting, it's recommended to use a dilution range of 1:500-1:2000. IHC staining can be performed using a dilution range of 1:50-1:200, while IF/ICC staining can be performed using a dilution range of 1:100-1:500. For ELISA assays using peptides, a dilution range of 1:20000-1:40000 is typically used. These dilution ranges will help ensure optimal results for your experiments.
Immunogen	A synthesized peptide derived from human IKK-beta.
MW	86kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was purified through peptide affinity chromatography. To ensure the highest level of purity and specificity, this method was chosen.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.
Tissue specificity	Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
Posttranslational modification	Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK as well as TBK1 and PRKCZ; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response. Phosphorylated by the IKK-related kinases TBK1 and IKBKE, which is associated with reduced CHUK/IKKA and IKBKB activity and NF-kappa-B-dependent gene transcription. Dephosphorylated at Ser-177 and Ser-181 by PPM1A and PPM1B.(Microbial infection) Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues.(Microbial infection) Monoubiquitination by TRIM21 is disrupted by Yersinia yopJ.Hydroxylated by PHD1/EGLN2, loss of hydroxylation under hypoxic conditions results in activation of NF-kappa-B.
Celluar localization	Cytosol;Nucleus;Plasma Membrane;
UniPort	O14920