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Rabbit Anti-HRAS Polyclonal Antibody#abs137096

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Description

Catalog-specification	Delivery time	USD price
abs137096-100ug	1-2 Weeks	301.0
abs137096-50ug	1-2 Weeks	201.0

Overview
catalog	abs137096
Other names	Ras is a well-known oncogene that plays a crucial role in cell signaling pathways. There are several types of Ras proteins, including C-Bas/Has, C-Ha Ras, C-Ha Ras1, and C-Has/Bas p21 protein. These proteins are involved in various cellular processes and have different functions. One of the widely studied Ras proteins is c-Ha-ras, also known as Ha-Ras or Ha-Ras1 proto-oncoprotein. It belongs to the Ras family of small GTP binding proteins and is encoded by the HRAS gene. This gene is highly conserved among species and is essential for normal cell growth and development. The c-Ha-Ras protein is characterized by its ability to bind to GTP and GDP. It has a GTPase activity, which allows it to hydrolyze GTP into GDP and release phosphate. This GTPase activity is crucial for the regulation of Ras signaling, as it controls the switch between the active GTP-bound form and the inactive GDP-bound form. The activation of the c-Ha-Ras oncogene is associated with a variety of human cancers. Mutations in the HRAS gene can lead to the constitutive activation of the protein, disrupting normal cellular signaling and promoting uncontrolled cell growth. For example, the v-Ha-ras oncogene, derived from the Harvey rat sarcoma viral oncogene, is a mutant form of c-Ha-Ras that is highly oncogenic. In addition to c-Ha-Ras, there are other members of the Ras protein family, such as K-ras and N-ras. These proteins share similar structural and functional characteristics, but they are encoded by different genes. They also play important roles in cell signaling and are frequently mutated in human cancers. Overall, Ras proteins are critical regulators of cell growth and differentiation. Their aberrant activation can contribute to the development of cancer, and they represent important therapeutic targets for the treatment of Ras-driven malignancies.
Source	Rabbit
Specificity	The HRAS Antibody is capable of detecting the total endogenous levels of HRAS. To create a similar content, one can state that the antibody has the ability to identify the entirety of HRAS present within an organism.
Species Reactivity	Human
Predictive reaction species	Chicken
Antigen	HRAS
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human HRAS.
MW	21 kDa
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography utilizing SulfoLink™ Coupling Resin was employed to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151).
Tissue specificity	Widely expressed.
Posttranslational modification	Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation.The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation.Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).
Celluar localization	Cytosol;Golgi apparatus;Nucleus;Plasma Membrane;
UniPort	P01112