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Rabbit Anti-HNF-4A Polyclonal Antibody#abs137075

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Description

Catalog-specification	Delivery time	USD price
abs137075-100ug	1-2 Weeks	301.0
abs137075-50ug	1-2 Weeks	201.0

Overview
catalog	abs137075
Other names	Hepatic nuclear factor 4 alpha (HNF4α), also known as hepatocyte nuclear factor 4 alpha or HNF4, is a transcription factor that plays a crucial role in regulating gene expression in the liver and pancreas. It is encoded by the HNF4A gene and belongs to the nuclear receptor subfamily 2 group A member 1. HNF4α, often referred to as HNF4, is involved in the development and function of hepatocytes and pancreatic beta cells. It controls the expression of genes related to glucose and lipid metabolism, as well as bile acid synthesis. Mutations in the HNF4A gene have been linked to maturity-onset diabetes of the young (MODY) type 1, a form of diabetes characterized by early-onset and impaired insulin production. HNF4α is also known by different aliases such as HNF4a7, HNF4a8, HNF4a9, and HNF4alpha10/11/12. It is a pivotal transcription factor in maintaining the normal physiology of the liver and pancreas. The alternative symbol TCF-14 or TCF14 is occasionally used to denote this factor, which stands for transcription factor 14 or transcription factor HNF-4. However, it should not be confused with the Tcf4 gene, which encodes transcription factor 4, a distinct protein unrelated to HNF4α. In conclusion, HNF4α is a crucial transcription factor involved in liver and pancreatic function, and its genetic mutations can lead to certain types of diabetes. This protein is also referred to by various other names, including HNF4 and TCF14, highlighting its significance in gene regulation and metabolic processes.
Source	Rabbit
Specificity	The HNF-4A Antibody is able to detect the total amount of endogenous HNF-4A present in the sample. This means that it is specifically designed to identify any changes in the levels of HNF-4A that may occur, enabling researchers to gain a deeper understanding of its function and role in various biological processes. By using this antibody, scientists can study HNF-4A with greater accuracy and precision, leading to enhanced insights into its potential therapeutic uses.
Species Reactivity	Human;Mouse;Rat
Antigen	HNF-4A
Application	You can use this protein assay in a variety of applications, ranging from Western blotting at 1:1000-3000 dilutions to IF/ICC at 1:100-1:500 dilutions. For ELISA using a peptide, the recommended dilution is between 1:20000 to 1:40000. Regardless of the method you choose, you can expect high-quality results with this protein assay.
Immunogen	A synthesized peptide derived from human HNF-4A.
MW	53 kDa
Properties
Concentration	1mg/ml
purification	The SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography to purify the antiserum. This process effectively purified the antiserum and made it suitable for further use.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage conditions, it is recommended to keep the product at a temperature of -20 °C for up to one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this may affect its stability and functionality over time. Therefore, it is crucial to handle and store the product with care to maintain its quality and effectiveness.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.
Posttranslational modification	Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA.Acetylation at Lys-458 lowers transcriptional activation by about two-fold.
Celluar localization	Nucleus;
UniPort	P41235