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Rabbit Anti-HFE2 Polyclonal Antibody#abs136802

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Description

Catalog-specification	Delivery time	USD price
abs136802-100ug	1-2 Weeks	301.0
abs136802-50ug	1-2 Weeks	201.0

Overview
catalog	abs136802
Description	This particular gene plays a role in iron metabolism and is implicated in various aspects of its regulation. It is believed to be involved in the signaling pathway that activates hepcidin, or potentially acts as a regulator of hepcidin expression. Furthermore, it could potentially serve as the cellular receptor for hepcidin. Different variations of this gene have been identified, resulting in the production of diverse isoforms. Mutations in this gene are responsible for hemochromatosis type 2A, also known as juvenile hemochromatosis (JH). JH is an inherited disorder characterized by excessive accumulation of iron, leading to conditions like hypogonadotrophic hypogonadism, liver fibrosis or cirrhosis, and cardiomyopathy. This condition typically manifests before the age of 30. To summarize, this gene has a significant role in iron metabolism and its dysfunctions can lead to the development of JH, a disorder marked by early-onset iron overload and associated complications.
Other names	The genes 2310035L15Rik and 5230400G09Rik, along with the molecular entities AI414844, AI789733, and DL M, are all associated with hemochromatosis, a condition characterized by excessive iron absorption. Two types of hemochromatosis, type 2A and type 2B, are caused by defects in the HFE2A gene, which encodes the hemochromatosis type 2 protein or hemojuvelin. Hemochromatosis type 2 is also known as juvenile hemochromatosis due to its onset in early adulthood. Another gene, RGM C, encodes the repulsive guidance molecule C and is considered a member of the RGM domain family. Overall, these genes and molecules play crucial roles in regulating iron homeostasis and preventing iron overload in the body.
Source	Rabbit
Specificity	The HFE2 Antibody is capable of detecting total HFE2 levels in an endogenous state. The content can be rearranged to convey the same information, stating that the HFE2 Antibody is designed to identify the complete amount of HFE2 present naturally within a sample.
Species Reactivity	Human;Mouse;Rat
Antigen	HFE2
Application	For Western blotting, the recommended dilution range is between 1:500 and 1:2000. For immunohistochemistry, a dilution range of 1:50 to 1:200 is advised. Meanwhile, for ELISA using peptide, a dilution range of 1:20000 to 1:40000 is suggested. These dilution ranges are important to follow in order to ensure accurate and reliable results in the respective assays.
Immunogen	A synthesized peptide derived from human HFE2.
MW	45kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Acts as a bone morphogenetic protein (BMP) coreceptor. Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis.
Tissue specificity	Adult and fetal liver, heart, and skeletal muscle.
Posttranslational modification	Autocatalytically cleaved at low pH; the two chains remain linked via two disulfide bonds. Also proteolytically processed by TMPRSS6, several fragments being released in the extracellular space; regulates HFE2 activity in BMP signaling and thefore iron homeostasis.
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	Q6ZVN8