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Rabbit Anti-HCLS1 Polyclonal Antibody#abs136408

Description

Catalog-specification	Delivery time	USD price
abs136408-100ug	1-2 Weeks	301.0
abs136408-50ug	1-2 Weeks	201.0

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Overview
catalog	abs136408
Description	HS1, also known as HCLS1, LckBP1, or p75, is a remarkable protein kinase substrate found exclusively in hematopoietic tissues and cells (1,2). Comprised of four cortactin repeats and a single SH3 domain (2), HS1 acts as an intracellular protein that undergoes phosphorylation upon activation of immune receptors, leading to its recruitment to the immune synapse (3-5). This phosphorylation event plays a vital role in regulating actin dynamics and creating docking sites for various signaling molecules, including Vav1 and PLCγ1 (6). Notably, HS1 also exerts significant influence on platelet activation (7). In summary, HS1 emerges as a fascinating protein kinase substrate expressed solely in hematopoietic tissues and cells (1,2). Its structure encompasses four cortactin repeats and a single SH3 domain (2). Notably, upon immune receptor activation, HS1 becomes phosphorylated, aiding its recruitment to the immune synapse (3-5). This phosphorylation process is pivotal in orchestrating actin dynamics and generating docking sites for crucial signaling molecules, like Vav1 and PLCγ1 (6). Furthermore, HS1 also exhibits a crucial role in platelet activation (7).
Other names	CTTNL, also known as Cortactin-like, HS1, HCLS1, or Hematopoietic cell-specific Lyn substrate 1, is a protein that is specific to hematopoietic lineage cells. It is also known as p75, HCLS1_HUMAN, LckBP1, or OTTHUMP00000215180. CTTNL is a substrate for the protein tyrosine kinase Lyn, and it has been implicated in signal transduction pathways that are mediated by Src family kinases. It plays an important role in regulating the actin cytoskeleton and cellular adhesion, and it is therefore important for cell motility and migration. CTTNL is also involved in immune responses, and its expression is upregulated in response to various stimuli, such as cytokines and antigens. Overall, CTTNL is a multifunctional protein that is important for the function of hematopoietic cells and immune responses.
Source	Rabbit
Specificity	The antibody HCLS1 is capable of detecting the total endogenous levels of HCLS1 protein. Rephrasing it using a different language model: The detection of endogenous levels of total HCLS1 is feasible with the use of the HCLS1 antibody.
Species Reactivity	Human;Mouse;Rat
Antigen	HCLS1
Application	The recommended dilutions for using this antibody in different applications are as follows: for Western blotting, the suggested dilution range is between 1:500 to 1:2000; for immunohistochemistry, it is recommended to use this antibody at a dilution range of 1:50 to 1:200; and for ELISA (peptide), the antibody should be diluted at a range of 1:20000 to 1:40000. These dilutions have been optimized for the best performance of the antibody in various applications. It is important to optimize the dilution for each experiment to achieve the best results.
Immunogen	A synthesized peptide derived from human HCLS1.
MW	54kDa
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography using SulfoLink™ Coupling Resin was employed for the purification of the antiserum. Let's create a distinct version of the content while preserving the original information.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
Tissue specificity	Expressed only in tissues and cells of hematopoietic origin.
Posttranslational modification	Phosphorylated by FES (By similarity). Phosphorylated by LYN, FYN and FGR after cross-linking of surface IgM on B-cells. Phosphorylation by LYN, FYN and FGR requires prior phosphorylation by SYK or FES.
Celluar localization	Cytosol;Mitochondrion;Nucleus;Plasma Membrane;
UniPort	P14317