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Rabbit Anti-H3K14me3 Polyclonal Antibody#abs136765

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Description

Catalog-specification	Delivery time	USD price
abs136765-100ug	1-2 Weeks	301.0
abs136765-50ug	1-2 Weeks	201.0

Overview
catalog	abs136765
Description	In eukaryotes, histones play a crucial role in the nucleosome structure of the chromosomal fiber. They are basic nuclear proteins and are responsible for the organization of DNA strands into nucleosomes consisting of DNA wrapped around a histone octamer. The histone octamer comprises pairs of each of the four core histones, namely H2A, H2B, H3, and H4. This structure of chromatin further gets compacted by linking the DNA between nucleosomes through a linker histone, H1, to form higher-order chromatin structures. The gene that encodes a member of the histone H3 family is intronless and lacks polyA tails in its transcripts. Instead, these transcripts contain a palindromic termination element. Interestingly, this gene is independently located from the other histone H3 genes that are a part of the histone gene cluster situated on chromosome 6p22-p21.3.
Other names	The H3 histone family is a group of proteins encoded by a cluster of histone genes located on chromosome 6 in humans. This family includes members such as H3.1, H3.2, and H3.3A, which are known for their important roles in chromatin structure and gene regulation. Other members of the family include H3E, H3F3, and H3FK, among others. These proteins have been shown to be involved in various cellular processes, including DNA replication and repair, transcriptional regulation, and cell cycle progression. Interestingly, some members of this family, such as Histone H3.3 and H3F1K, are specifically associated with histone turnover and replacement, further emphasizing the crucial functions of these proteins in maintaining proper chromatin structure and cellular functionality. In addition, many of the histone family members have been implicated in various diseases, including cancer and neurological disorders. As such, understanding the functions and regulation of these proteins could have important implications for the development of novel therapeutics for these conditions. Overall, the H3 histone family represents a diverse group of proteins with important roles in chromatin dynamics, gene regulation, and disease pathology.
Source	Rabbit
Specificity	H3K14me3 Antibody detects endogenous levels of total H3K14me3.
Species Reactivity	Human;Mouse;Rat
Antigen	H3K14me3
Application	WB 1:500-1:2000, IHC 1:50-1:200, IF 1:20-1:50, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthetic peptide of human H3K14me3.
MW	15kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Tissue specificity	Expressed in testicular cells.
Posttranslational modification	Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability (By similarity).Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters (By similarity).Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication (By similarity).Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MAP3K20 isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin (By similarity).Ubiquitinated.Lysine deamination at Lys-5 (H3K4all) to form allysine is mediated by LOXL2. Allysine formation by LOXL2 only takes place on H3K4me3 and results in gene repression (By similarity).
Celluar localization	Extracellular region or secreted;Nucleus;
UniPort	Q16695