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Rabbit Anti-H3K14me2 Polyclonal Antibody#abs136764

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. We apologize for any convenience caused. Western blot analysis of extracts of HeLaand H3 protein, using H3K14me2 antibody. This product is for...

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Description

Catalog-specification	Delivery time	USD price
abs136764-100ug	1-2 Weeks	301.0
abs136764-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. We apologize for any convenience caused.

Overview
catalog	abs136764
Description	The structure of the chromosomal fiber in eukaryotes relies on histones, which are fundamental nuclear proteins. These histones form nucleosomes, where approximately 146 bp of DNA wraps around a histone octamer made up of pairs of four core histones (H2A, H2B, H3, and H4). To further compact the chromatin fiber into higher order structures, the DNA between nucleosomes interacts with a linker histone called H1. Unlike other genes in the histone gene cluster on chromosome 6p22-p21.3, this particular gene encoding a member of the histone H3 family has no introns. Interestingly, the transcripts of this gene do not possess polyA tails but instead contain a palindromic termination element.
Other names	There are a multitude of identifiers for histones, specifically within the H3 family. Some of the most common identifiers for H3 histones include H3.3A, H3E, H3.1, H3F3, and H3FL. Additionally, there are various clusters of H3 histone genes, such as the HIST1 cluster, which includes members A-J, and the Histone gene cluster 1 that includes members A-D and F-J. In addition to these identifiers, there are numerous other variations and combinations, such as H3 histone family members B-K, H3 histone family member T, H3 histone family 3A, and H3FA-H3FK. There are also histone 1 variants, including H3a-j and histone cluster 1 variants, including H3a-j. In terms of the physical properties of H3 histones, there are various types, including H3.1, H3.2, and H3.3, and they play important roles in packaging DNA within the nucleus of cells. While there are numerous variations and identifiers for H3 histones, they all share an important commonality in their crucial function within the cell.
Source	Rabbit
Specificity	H3K14me2 Antibody detects endogenous levels of total H3K14me2.
Species Reactivity	Human;Mouse;Rat
Antigen	H3K14me2
Application	WB 1:500-1:2000, IHC 1:50-1:200, IF 1:20-1:50, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthetic peptide of human H3K14me2.
MW	15kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Tissue specificity	Expressed in testicular cells.
Posttranslational modification	Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability (By similarity).Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters (By similarity).Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication (By similarity).Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MAP3K20 isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin (By similarity).Ubiquitinated.Lysine deamination at Lys-5 (H3K4all) to form allysine is mediated by LOXL2. Allysine formation by LOXL2 only takes place on H3K4me3 and results in gene repression (By similarity).
Celluar localization	Extracellular region or secreted;Nucleus;
UniPort	Q16695