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Rabbit Anti-GROgamma Polyclonal Antibody#abs138074

Description

Catalog-specification	Delivery time	USD price
abs130074-50ug	1-2 Weeks	201.0
abs130074-100ug	1-2 Weeks	301.0

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Overview
catalog	abs130074
Description	Connexin-43 is an important integral membrane protein belonging to the alpha-type (group II) subfamily of the connexin family. These proteins form hexamers that combine to form connexons, which are then assembled into gap junctions. These gap junctions allow for the diffusion of small molecules and materials between adjacent cells. One significant function of connexin-43 is its participation in the recycling of potassium to the cochlear endolymph, suggesting a crucial role in the physiology of hearing. Overall, connexin-43 is a critical protein that contributes to proper cell-to-cell communication in various physiological processes.
Other names	Connexin 43, also known as Cx 43 or Cx43, is a protein present in the human body. It is also referred to as CXA1_HUMAN, DFNB38, or Gap junction 43 kDa heart protein. Its function involves forming gap junctions, which allow the exchange of molecules and ions between adjacent cells. Connexin 43 is specifically found in the heart and is crucial for proper cardiac functioning. Mutations in this protein are associated with conditions like syndactyly type 3 (SDTY3), oculodentodigital dysplasia (ODD), oculodentodigital dysplasia with microcornea (ODDD), and oculodentodigital dysplasia with osteodysplasty (ODOD). Understanding the role and structure of connexin 43 is important in studying cellular communication and its impact on various genetic disorders.
Source	Rabbit
Specificity	Connexin 43 antibody detects endogenous levels of total Connexin 43.
Species Reactivity	Human;Mouse;Rat
Antigen	Connexin 43
Application	For Western blotting, the recommended dilution range is between 1:500 to 1:3000. In regards to immunohistochemistry, the ideal dilution range is between 1:50 to 1:200. As for immunofluorescence or immunocytochemistry, the range is between 1:100 to 1:500. Finally, for ELISA (peptide), a dilution of 1:20000-1:40000 is recommended. Please note that these are mere guidelines and optimal dilution conditions may vary depending on the specific experiment conditions and application.
Immunogen	A human Connexin 43-sourced peptide that has been synthesized is available. The peptide has been created based on the original text information and is highly similar.
MW	43kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, peptide affinity chromatography was utilized to purify the antiserum in question.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity).
Tissue specificity	Expressed in the heart and fetal cochlea.
Posttranslational modification	Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity (By similarity). Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation (By similarity).Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2.S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication.
Celluar localization	Endoplasmic reticulum;Endosome;Extracellular region or secreted;Golgi apparatus;Lysosome;Mitochondrion;Plasma Membrane;
UniPort	P17302