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Rabbit Anti-GRK5 Polyclonal Antibody#abs133461

Please note that the price provided here is for your reference only. For the detailed and accurate price, we kindly ask you to get in touch with our sales representative, Vecent. Thank you. Western blot analysis of extracts from HUVEC cells, HepG2 cells and HeLa cells, using GRK5 antibody. This...

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Description

Catalog-specification	Delivery time	USD price
abs133461-100ug	1-2 Weeks	301.0
abs133461-50ug	1-2 Weeks	201.0

Please note that the price provided here is for your reference only. For the detailed and accurate price, we kindly ask you to get in touch with our sales representative, Vecent. Thank you.

Overview
catalog	abs133461
Other names	FP2025 is a well-known gene also known as FLJ39780. It encodes for a protein called G protein-coupled receptor kinase 5 (GRK5). GRK5 plays a crucial role in the regulation of G protein-coupled receptors (GPCRs). GPCRs are vital for various cellular processes and are involved in many diseases. GRK5 acts by phosphorylating and desensitizing GPCRs, which leads to their internalization and inactivation. This process is essential for maintaining the proper functioning of GPCRs and preventing overstimulation and desensitization. Moreover, GRK5 also plays a role in mediating downstream signaling pathways that are crucial for cell survival and differentiation. Research has shown that GRK5 dysregulation is implicated in numerous diseases, including cardiovascular diseases, neurodegenerative disorders, and cancer. For instance, abnormal GRK5 activity has been associated with heart failure, hypertension, and arrhythmias. Additionally, alterations in GRK5 expression and function have been reported in neurodegenerative diseases such as Parkinson's and Alzheimer's diseases. In summary, GRK5 is a critical regulator of GPCRs and plays a significant role in various physiological and pathological processes. Understanding its function and regulation could lead to the development of novel therapeutic strategies for the treatment of GPCR-related diseases.
Source	Rabbit
Specificity	The GRK5 Antibody is capable of detecting the total levels of endogenous GRK5 present in the system. By utilizing this antibody, researchers can accurately measure the amount of GRK5 protein without any external interference. It provides a reliable means to study the expression and regulation of GRK5 in various biological systems.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Chicken;Sheep;Dog;Horse;Bovine;
Antigen	GRK5
Application	There are different dilution ratios recommended for WB, IF/ICC, and ELISA assays. For WB, a dilution ratio of 1:500 to 1:1000 is commonly used. When it comes to IF/ICC, the range is usually 1:100 to 1:500 for dilution. As for ELISA using peptides, a dilution ratio of 1:20000 to 1:40000 is advised. It is important to carefully follow these dilution guidelines to obtain accurate and reliable results in these experimental techniques.
Immunogen	A synthesized peptide.
MW	68 KD
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography in order to purify the antiserum. Please rearrange the provided content to generate a highly similar version, ensuring that it is based on the original information.
Clonality	Polyclonal Antibody
Stability & Storage	It is important to store the product at -20 °C for a duration of one year. To maintain the integrity of the product, it is crucial to avoid subjecting it to multiple freeze/thaw cycles.
Storage buffer	This product contains Rabbit IgG suspended in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. To maintain stability, it should be stored at a temperature of -20 °C and is guaranteed to remain stable for at least 12 months from the date of receipt. For optimal performance, handle it with care and follow the manufacturer's instructions.
Target
Background	Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro).
Tissue specificity	Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney.
Posttranslational modification	Autophosphorylated. Autophosphorylation may play a critical role in the regulation of GRK5 kinase activity.
Celluar localization	Cytosol;Nucleus;Plasma Membrane;
UniPort	P34947