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Rabbit Anti-GPR77 Polyclonal Antibody#abs133141

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Description

Catalog-specification	Delivery time	USD price
abs133141-100ug	1-2 Weeks	301.0
abs133141-50ug	1-2 Weeks	201.0

Overview
catalog	abs133141
Description	The anaphylatoxin receptors are responsible for binding to chemotactic and inflammatory peptides such as C5a, C4a and C3a, as well as their desarginated derivatives. These receptors have the ability to weakly couple with G(i)-mediated signaling pathways.
Other names	The C5a anaphylatoxin chemotactic receptor C5L2, also known as G protein coupled receptor 77 or GPR77, is a protein that plays an important role in the immune system. It is encoded by the E030029A11Rik gene and is involved in the chemotaxis and activation of leukocytes, the recruitment of inflammatory cells, and the regulation of immune responses. C5L2 is closely related to another C5a receptor, C5AR, but has a distinct function and signaling pathway. Despite its name, C5L2 does not bind C5a anaphylatoxin with high affinity and has been proposed to act as a negative regulator of C5a-mediated responses.
Source	Rabbit
Specificity	Total GPR77 can be detected by the GPR77 antibody, which has the ability to identify endogenous levels of this protein.
Species Reactivity	Human
Antigen	GPR77
Application	The recommended dilution for Western blot is between 1:500 to 1:2000, while for Immunohistochemistry it is 1:50 to 1:200. For ELISA using peptide, the dilution range recommended is 1:20000 to 1:40000. It is important to follow these recommended dilutions to achieve optimal results in your experiments. To ensure accuracy, always use high-quality reagents and follow the correct protocol for your specific assay.
Immunogen	A synthesized peptide.
MW	36 kDa
Properties
Concentration	1mg/ml
purification	Utilizing SulfoLink™ Coupling Resin, we performed peptide affinity chromatography to purify the antiserum. This process yielded a highly purified product that was free from impurities.
Clonality	Polyclonal Antibody
Stability & Storage	Keep the product stored at a temperature of -20 °C for a duration of one year, making sure to prevent any repeated freezing and thawing cycles.
Storage buffer	In order to maintain its stability and effectiveness, Rabbit IgG is stored in a solution containing phosphate buffered saline, with a pH of 7.4, 150mM NaCl, and 0.02% sodium azide. To prevent freezing and promote longevity, the solution is also enriched with 50% glycerol. This mixture ensures the successful application and usage of Rabbit IgG in various experiments and assays.
Target
Background	Receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides and also for their dearginated forms ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to G(i)-mediated signaling pathways.
Tissue specificity	Frontal cortex, hippocampus, hypothalamus, pons and liver.
Celluar localization	Plasma Membrane;
UniPort	Q9P296