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Rabbit Anti-GFI1B Polyclonal Antibody#abs132788

Please note that the given price is for your reference only. For the detailed price, kindly reach out to our seller, Vecent. Western blot analysis of GFI1B expression in Jurkat cell lysate;,The lane on the left is treated with the antigen-specific peptide. This product is for research use only,...

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Description

Catalog-specification	Delivery time	USD price
abs132788-50ug	1-2 Weeks	201.0
abs132788-100ug	1-2 Weeks	301.0

Please note that the given price is for your reference only. For the detailed price, kindly reach out to our seller, Vecent.

Overview
catalog	abs132788
Description	GFI1B is a critical transcriptional regulator that plays a crucial role in the development and differentiation of erythroid and megakaryocytic lineages. Its function as a transcriptional repressor or activator depends on the specific promoter and cell type it acts upon. In certain contexts, GFI1B represses the activity of promoters such as SOCS1 and SOCS3, thereby regulating cytokine signaling pathways. Working in collaboration with GATA1, GFI1B helps repress the transcription of target genes like BCL2L1, an apoptosis regulator. Suppression of GFI1B in leukemic cell lines leads to a significant increase in the rate of apoptosis. Additionally, GFI1B inhibits the down-regulation of oncogenes MYC and MYB, as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in myelomonocytic cells treated with IL6. Notably, GFI1B also represses the expression of GATA3 in T-cell lymphomas, thus exerting an inhibitory effect on GATA1-mediated transcription. As GATA1 is also involved in the regulation of GFI1B transcription in erythroid cells, both GATA1 and GFI1B participate in a feedback regulatory pathway that controls the expression of the GFI1B gene. Furthermore, GFI1B interacts with its own promoter and gamma-satellite DNA, leading to auto-repression of its own expression. It also plays a role in modifying histone methylation patterns by recruiting histone methyltransferase to the promoters of target genes. Moreover, GFI1B contributes to the formation of heterochromatin. In summary, GFI1B is an essential proto-oncogenic transcriptional regulator that is indispensable for the proper development and differentiation of erythroid and megakaryocytic lineages. Its versatile role as a transcriptional repressor or activator, its involvement in apoptotic pathways, and its ability to modulate gene expression through interactions with various factors make GFI1B a crucial player in cellular processes.
Other names	The GFI1B protein, also known as GFI 1B or Growth factor independent 1B protein, is a transcription repressor that plays a role in regulating the growth and development of cells. It is encoded by the GFI1B_HUMAN gene and is also referred to as Growth factor independent 1B transcription repressor or Growth factor independent protein 1B. The GFI1B protein has been identified as a potential regulator of CDKN1A, a gene involved in cell cycle regulation and proliferation. It is also associated with CDKN1A translocated in CML, a form of leukemia. Additionally, it is implicated in the development of certain cancers. The GFI1B protein contains zinc finger domains, which are known to mediate DNA binding and transcriptional regulation. Its function is crucial for maintaining the balance between cell growth and differentiation. This protein is encoded by several alternative splice variants, including OTTHUMP00000022443, OTTHUMP00000022444, and OTTHUMP000000235527. It is known to be involved in the translocation associated with chronic myeloid leukemia, a type of blood cancer. In summary, GFI1B is an essential protein involved in regulating cell growth and development. It has a potential role in the regulation of CDKN1A and is associated with leukemia. Its zinc finger domains play a critical role in its function as a transcriptional regulator.
Source	Rabbit
Specificity	Detecting endogenous levels of total GFI1B is possible using the GFI1B antibody.
Species Reactivity	Human;Mouse
Predictive reaction species	Pig;Sheep;Dog;Horse;Bovine
Antigen	GFI1B
Application	WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide.
MW	43 kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.
Tissue specificity	Expressed in bone marrow and fetal liver, but also detectable in fetal spleen, fetal thymus, and testes. Detected in hematopoietic stem cells, erythroblasts, and megakaryocytes. Overexpressed in bone marrow of patients with erythroleukemia and megakaryocytic leukemia as well as in their corresponding leukemic cell lines, and markedly repressed in severe aplastic anemia (SAA).
Posttranslational modification	Methylation at Lys-8 in the SNAG domain seems required for the recruitment of the corepressor complex.
Celluar localization	Nucleus;
UniPort	Q5VTD9