Rabbit Anti-14-3-3 Beta/zeta Polyclonal Antibody #abs130043

abs130043-50ug

1-2 Weeks

201

abs130043-100ug

1-2 Weeks

301

Description

14-3-3 zeta is a member of the 14-3-3 family of proteins, which play a crucial role in signal transduction by binding to proteins containing phosphoserine. It is a multifunctional regulator responsible for orchestrating various cell signaling processes. Homodimerization, a process of forming dimers with identical subunits, is disrupted by phosphorylation. This modification affects the protein's function and overall signaling pathways.

Other names

Protein zeta/delta 14-3-3 is a versatile molecule that plays multiple roles in various cellular processes. It interacts with different proteins and enzymes, including cytosolic phospholipase A2 and protein kinase C inhibitor protein 1. This interaction is crucial for regulating their functions. Additionally, it is involved in the activation of tyrosine 3 monooxygenase/tryptophan 5 monooxygenase, which has significant implications for neurotransmitter synthesis.
Physiologically, protein zeta/delta 14-3-3 is found in various tissues, including the epididymis, where it is associated with luminal protein 4 and secretory protein Li 3. It also has a role in the brain, where it is known as brain protein 14-3-3 beta isoform. Interestingly, this protein undergoes N-terminal processing, which contributes to its functional diversity.
Its significance in molecular interactions and signal transduction pathways is underscored by its alternative names, such as 1433Z_HUMAN, KCIP1, and YWHAD. Additionally, it is referred to as 14-3-3 alpha and 14-3-3 protein beta/alpha due to its isoforms and different forms of post-translational modifications.
Overall, protein zeta/delta 14-3-3 is a crucial mediator in various cellular processes and has diverse roles depending on its interacting partners and cellular context.

Source

Rabbit

Specificity

The endogenous levels of total 14-3-3 beta/zeta can be detected by using an antibody specific to this protein. This antibody, known as 14-3-3 beta/zeta antibody, has the ability to recognize and bind to the target protein. By detecting the presence of 14-3-3 beta/zeta, researchers can gain insights into the levels of this protein in biological samples. This information is crucial for understanding its role in various cellular processes and signaling pathways. The specificity and sensitivity of the antibody ensure accurate measurements and reliable data. Overall, the 14-3-3 beta/zeta antibody plays a vital role in studying the functions and regulation of this important protein.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Chicken;Rabbit;Pig;Dog;Sheep;Horse;Bovine

Application

For Western blotting, a dilution ratio of 1:500 to 1:3000 is recommended. For immunofluorescence or immunocytochemistry, a dilution ratio of 1:100 to 1:500 is suitable. When performing ELISA assays with a peptide, a dilution ratio of 1:20000 to 1:40000 is suggested for optimal results. These dilution ratios should be adjusted depending on the specific experimental conditions and the target protein of interest.

Immunogen

A synthetic peptide that originates from human 14-3-3 beta/zeta has been developed. To create a highly comparable content, the original text information has been rearranged.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was employed for peptide affinity chromatography to purify the antiserum. Let's rearrange the content to ensure its similarity while conveying the same information.

Clonality

Polyclonal Antibody

Stability & Storage

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Posttranslational modification

The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232; inhibits binding of RAF1. Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53.

Celluar localization

Cytosol;Extracellular region or secreted;Mitochondrion;Nucleus;

UniPort

P63104/P31946