Rabbit Anti-GAK Polyclonal Antibody #abs130253

abs130253-50ug

1-2 Weeks

201

abs130253-100ug

1-2 Weeks

301

Description

CDK5 and cyclin G are associated with GAK Associates. Its function appears to mimic that of auxilin, acting in the uncoating process of clathrin-coated vesicles alongside Hsc70 in cells other than neurons. Throughout the cell cycle, its expression shows slight oscillations, reaching its highest level during the G1 phase. GAK Associates is classified under the protein kinase superfamily.

Other names

GAK, also known as Cyclin-G-associated kinase or Auxilin 2, is an important protein involved in cellular processes such as endocytosis and cell cycle regulation. It belongs to the family of serine/threonine kinases and has been identified as a critical regulator of clathrin-mediated endocytosis, which is essential for the uptake of extracellular materials such as nutrients, signaling molecules and pathogens. GAK has also been shown to be involved in the regulation of protein degradation, cell division and apoptosis.
The structure of GAK consists of several domains including a kinase domain, a cyclin-binding domain and a Hsc70-binding domain. Its activity is regulated by various mechanisms including phosphorylation, interaction with cyclins and binding to adaptor proteins such as Hsc70 and auxilin. As a key mediator of endocytic events, GAK has been implicated in several diseases including cancer, neurodegenerative disorders and viral infections.
In summary, GAK or Cyclin-G-associated kinase plays a crucial role in cellular processes such as endocytosis and cell cycle regulation. With its multifunctional domains and regulatory mechanisms, GAK has emerged as an important therapeutic target for various diseases.

Source

Rabbit

Specificity

The GAK antibody is capable of identifying the total GAK present in the body, specifically detecting its endogenous levels. To maintain the essence of the original content, the rearranged version would be as follows:
Endogenous levels of total GAK can be identified using the GAK antibody. This antibody is designed to specifically detect the presence of GAK in the body.

Species Reactivity

Human;Mouse

Application

Western blot (WB): The dilutions used for WB range from 1:500 to 1:3000. For immunofluorescence (IF) or immunocytochemistry (ICC), the recommended dilutions are 1:100 to 1:500. For ELISA using peptide antigens, a dilution of 1:20000 to 1:40000 is suggested.

Immunogen

A synthesized peptide derived from human GAK

Properties

Concentration

1mg/ml

Purification

Affinity-chromatography

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality of the product, it is recommended that you store it at a temperature of -20°C for a period of one year. Be sure to avoid exposing it to repeated cycles of freezing and thawing, as this can compromise its integrity. By following these guidelines, you can ensure that the product remains in optimal condition for its intended use.

Storage buffer

The rabbit IgG is preserved in a solution of phosphate buffered saline with a pH of 7.4. It is mixed with 150mM NaCl, 0.02% sodium azide, and 50% glycerol for proper storage. This product should be kept at a temperature of -20 °C to maintain its stability. It can be stored for up to 12 months from the date of receipt. Please note that the content generated should be significantly different from the original text information.

Target

Background

This protein is known to associate with CDK5 and cyclin G. It functions as an auxilin homolog that participates in the uncoating of clathrin-coated vesicles by Hsc70 in cells other than neurons. Moreover, its expression level slightly oscillates during the course of the cell cycle, with a peak observed during the G1 phase.

Tissue specificity

Ubiquitous. Highest in testis.

Celluar localization

Cytosol;Golgi apparatus;

UniPort

O14976