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Rabbit Anti-FUT3 Polyclonal Antibody#abs141147

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Description

Catalog-specification	Delivery time	USD price
abs141147-100ug	1-2 Weeks	301.0
abs141147-50ug	1-2 Weeks	201.0

Overview
catalog	abs141147
Other names	Fucosyltransferase III, also known as alpha-(1,3/1,4)-fucosyltransferase or Lewis blood group fucosyltransferase, is an enzyme that plays a crucial role in the modification of glycoproteins and glycolipids. It is encoded by the FUT3 gene and is commonly referred to as CD174 or FT3B. This enzyme is responsible for catalyzing the transfer of fucose molecules to specific sugar residues on the glycan chains of various target molecules. It specifically adds the fucose molecule to the galactose residue at positions 3 and 4, resulting in the synthesis of Lewis antigens, such as Lewis a and Lewis b. The Lewis antigens are commonly found on the cell surface and are known to be involved in various biological processes, including cell-cell recognition and adhesion. They have also been implicated in cancer progression and metastasis. Fucosyltransferase III is highly expressed in a variety of tissues, including the gastrointestinal tract, liver, and pancreas. Mutations in the FUT3 gene can lead to the absence or reduced expression of Lewis antigens, resulting in altered cell-surface properties and potential effects on disease susceptibility. Understanding the function and regulation of fucosyltransferase III is not only important for elucidating the biology of glycosylation but also for developing therapeutic strategies targeting glycan-mediated processes.
Source	Rabbit
Specificity	The FUT3 Antibody is capable of detecting total levels of endogenous FUT3. To create a closely related statement, the content can be rearranged to state that the antibody is able to identify the overall quantity of FUT3 naturally present within a sample.
Species Reactivity	Human;Mouse
Antigen	FUT3
Application	WB dilution range for this target is 1:500~1:1000, while for IHC it is 1:50~1:200. The dilution range for IF/ICC is 1:100-1:500, and for ELISA (peptide) it is 1:20000-1:40000. It is crucial to follow these dilution guidelines to obtain accurate and reliable results in your experiments.
Immunogen	A synthesized peptide.
MW	42 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was purified through peptide affinity chromatography. To ensure high quality and specificity, this purification process was carefully carried out with precision and accuracy. Through the utilization of advanced techniques and technology, the resulting product is of exceptional purity and potency.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the product's quality, it is advised to store it in a -20 °C environment for up to a year. It is recommended to prevent repetitive freezing and thawing cycles to maintain its stability.
Storage buffer	This product contains Rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4. It also includes 150mM of NaCl, 0.02% of sodium azide and 50% glycerol. The recommended storage temperature is -20 °C, and the product is stable for at least 12 months from the time of receipt.
Target
Background	May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le+) individuals have an active enzyme while Lewis-negative (Le-) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.
Tissue specificity	Highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver.
Celluar localization	Extracellular region or secreted;Golgi apparatus;
UniPort	P21217