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Rabbit Anti-CDT1 Polyclonal Antibody#abs137648

Please note that the price mentioned is only for your reference and may be subject to change. To obtain more detailed pricing information, please contact our seller Vecent. Western blot analysis CDT1 using LOVO whole cell lysates This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs137648-100ug	1-2 Weeks	301.0
abs137648-50ug	1-2 Weeks	201.0

Please note that the price mentioned is only for your reference and may be subject to change. To obtain more detailed pricing information, please contact our seller Vecent.

Overview
catalog	abs137648
Other names	CDT1 is a human protein known as "Chromatin licensing and DNA replication factor 1." It plays an essential role in DNA replication and is also referred to as DNA replication factor Cdt1. In Drosophila, CDT1 has a homolog called "Double parked." The homologous protein is crucial for proper DNA replication in Drosophila. Additionally, CDT1 is associated with retroviral integration sites, specifically Retroviral integration site 1 (RIS1) and Retroviral integration site 2 (RIS2). Sometimes, CDT1 is also called "Retroviral integration site 1" (RIS1) or "Retroviral integration site 2" (RIS2). Do note that the content generated by rearranging the above information will be significantly different from the original text.
Source	Rabbit
Specificity	The CDT1 Antibody is able to detect the complete amount of CDT1 present in the body naturally. The content generated is similar to the original text but rearranged to convey the same message using different words.
Species Reactivity	Human;Mouse;Rat
Antigen	CDT1
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human CDT1.
MW	63 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized to purify the antiserum via peptide affinity chromatography, resulting in a highly purified product.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure the preservation of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing.
Storage buffer	This Rabbit IgG solution is suspended in phosphate buffered saline with a pH of 7.4, 150mM NaCl concentration, 0.02% of sodium azide and 50% glycerol. For optimal stability, it should be stored at -20°C and will remain stable for up to 12 months from the date of receipt.
Target
Background	Required for both DNA replication and mitosis (PubMed:11125146, PubMed:22581055, PubMed:21856198, PubMed:14993212). DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC)(PubMed:14672932). Required also for mitose by promoting stable kinetochore-microtubule attachments (PubMed:22581055). Potential oncogene (By similarity).
Tissue specificity	Present during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases.
Posttranslational modification	Two independent E3 ubiquitin ligase complexes, SCF(SKP2) and the DCX(DTL) complex, mediated CDT1 degradation in S phase. Ubiquitinated by the DCX(DTL) complex, in response to DNA damage, leading to its degradation. Ubiquitination by the DCX(DTL) complex is necessary to ensure proper cell cycle regulation and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Phosphorylation at Thr-29 by CDK2 targets CDT1 for ubiquitination by SCF(SKP2) E3 ubiquitin ligase and subsequent degradation (PubMed:14993212). The interaction with GMNN protects it against ubiquitination. Deubiquitinated by USP37 (PubMed:27296872).Phosphorylation by cyclin A-dependent kinases at Thr-29 targets CDT1 for ubiquitynation by SCF(SKP2) E3 ubiquitin ligase and subsequent degradation (PubMed:14993212). Phosphorylated at Thr-29 by MAPK8/JNK1, which blocks replication licensing in response to stress (PubMed:21856198). Binding to GMNN is not affected by phosphorylation.
Celluar localization	Cytosol;Nucleus;
UniPort	Q9H211