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Rabbit Anti-eRF3a Polyclonal Antibody#abs140416

Please be informed that the mentioned price is only for your reference. For detailed pricing information, we kindly request you to get in touch with our sales representative, Vecent. Your cooperation is greatly appreciated. Western blot analysis eRF3a using 3T3 whole cell lysates This product is...

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Description

Catalog-specification	Delivery time	USD price
abs140416-100ug	1-2 Weeks	301.0
abs140416-50ug	1-2 Weeks	201.0

Overview
catalog	abs140416
Other names	The protein known as Anti Eukaryotic Release Factor 3a, also referred to as eRF3a or GSPT1, is a crucial component involved in the G1 to S phase transition of the cell cycle. This protein is homologous to the yeast protein GST1 and is responsible for mediating the GTP binding process in this transition. Its primary function is to regulate the release of eukaryotic release factor during translation termination, ensuring proper protein synthesis. G1 to S phase transition protein 1, or GST1, acts as a mediator in this important cellular event. Through rearranging the provided information, we understand the significance of eRF3a and its role in cell cycle regulation.
Source	Rabbit
Specificity	The eRF3a Antibody is capable of detecting the total amount of endogenous eRF3a present in a sample. A highly similar content can be generated by rephrasing the given information as follows: The detection of endogenous eRF3a levels can be achieved with the use of the eRF3a Antibody. The antibody is capable of recognizing eRF3a present in a sample, thereby allowing for the measurement of its total amount.
Species Reactivity	Human;Mouse
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Horse;Bovine;
Antigen	eRF3a
Application	WB 1:1000, IHC 1:200, ELISA (peptide) 1:20000-1:40000. Let's rearrange the information to present it in a slightly different manner. The dilution ratios for Western Blot (WB) and Immunohistochemistry (IHC) are 1:1000 and 1:200, respectively. For ELISA using peptides, the recommended dilution range is 1:20000 to 1:40000. Feel free to ask if you have any further questions.
Immunogen	A synthesized peptide derived from human eRF3a.
MW	55kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized to purify the antiserum through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the integrity of the product, it is recommended to keep it stored in a temperature-controlled environment at -20°C for up to one year. It is important to avoid subjecting it to multiple rounds of freezing and thawing, as this could compromise its quality over time. So make sure to follow these guidelines, and you'll be able to ensure the longevity of your product.
Storage buffer	This Rabbit IgG solution is formulated in a pH 7.4 phosphate buffered saline containing 150mM of NaCl and 0.02% of sodium azide. It is also supplemented with 50% glycerol to aid its conservation. To keep its quality intact, store it at a freezing point of -20 °C. This IgG solution is stable for 12 months from the date of receipt.
Target
Background	Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.
Celluar localization	Cytosol;
UniPort	P15170