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Rabbit Anti-EPOR Polyclonal Antibody#abs136628

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Description

Catalog-specification	Delivery time	USD price
abs136628-100ug	1-2 Weeks	301.0
abs136628-50ug	1-2 Weeks	201.0

Overview
catalog	abs136628
Other names	The EPO R, also known as EPO-R or epor, is a protein called Erythropoietin receptor. It is encoded by the EPOR_HUMAN gene and is synthesized as a precursor molecule. Another name for this receptor is MGC138358. It is crucial for mediating the effects of erythropoietin, a hormone that stimulates the production of red blood cells. The EPO R plays a vital role in regulating erythropoiesis, the process by which red blood cells are produced. Rearranging the given information, we can describe the EPO R as a protein receptor involved in responding to erythropoietin and promoting the generation of red blood cells in the human body.
Source	Rabbit
Specificity	The detection of endogenous levels of total EPOR can be achieved using EPOR Antibody. Please note that the generated content should be based on the original text information but presented in a different manner, and should not follow the conversational style of ChapGPT.
Species Reactivity	Human;Mouse;Rat
Antigen	EPOR
Application	The dilutions for Western Blot (WB) range from 1:500 to 1:2000, for Immunofluorescence (IF) and Immunocytochemistry (ICC) they range from 1:100 to 1:500, and for ELISA (peptide) it is from 1:20000 to 1:40000. This information provides guidance on the recommended dilutions for different experimental techniques. Please note that alternative dilution ranges may also be used depending on the specific experimental conditions and the desired level of sensitivity.
Immunogen	A synthetic peptide of human EPOR.
MW	55kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography in the purification process of the antiserum. To ensure a highly similar content based on the original information, the following content is generated with rearranged sentence structure.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is recommended to store it at -20 °C for a period of one year. It's important to avoid subjecting the product to repeated cycles of freezing and thawing.
Storage buffer	The Rabbit IgG is stored in a solution containing phosphate buffered saline at a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store it at a temperature of -20 °C to maintain its stability for up to 12 months from the date of receipt.
Target
Background	EPOR is the receptor responsible for recognizing and binding to erythropoietin, a hormone that plays a crucial role in promoting the proliferation and differentiation of erythroblasts. When erythropoietin binds to EPOR, it triggers the formation of EPOR dimers, which subsequently activate the JAK2/STAT5 signaling cascade. This signaling pathway plays a pivotal role in transmitting the erythropoietin-induced signals that promote erythroblast growth and maturation. In addition to STAT5 activation, EPOR can also activate other signal transducers such as STAT1 and STAT3 in certain cell types. Furthermore, EPOR has the capacity to activate the LYN tyrosine kinase, expanding its role in intracellular signaling events. This receptor and its associated signaling pathways are essential for the regulation of erythropoiesis, ensuring the production of an adequate number of functional red blood cells in the body.
Tissue specificity	Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells.
Posttranslational modification	On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site.Ubiquitination at Lys-281 mediates receptor internalization, whereas ubiquitination at Lys-453 promotes trafficking of activated receptors to the lysosomes for degradation (By similarity). Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	P19235