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Rabbit Anti-EPHB4 Polyclonal Antibody#abs131367

Description

Catalog-specification	Delivery time	USD price
abs131367-50ug	1-2 Weeks	201.0
abs131367-100ug	1-2 Weeks	301.0

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Overview
catalog	abs131367
Description	Ephrin-B2 receptor, a crucial player in differentiation and development, specifically binds to members of the ephrin-B family. Its involvement in mediating various events is well-established.
Other names	The human EphB4 gene codes for the protein called ephrin receptor EphB4, which belongs to the family of receptor tyrosine kinases. This protein is involved in cellular communication and plays a crucial role in various developmental processes. It is also known by other names, such as Hepatoma Transmembrane Kinase (HTK), MYK 1, and TYRO 11. The expression of EphB4 is observed in various tissues, including the nervous system, heart, and intestines. It binds to the ephrin-B ligands, and upon binding, activates signaling pathways that regulate cellular processes like cell migration and adhesion. Moreover, EphB4 signaling is known to be involved in angiogenesis, the formation of new blood vessels from pre-existing ones, which is crucial for tumor growth. Mutations in the EphB4 gene have been associated with various diseases, including cancer, cardiovascular disorders, and Alzheimer's disease. Therefore, understanding the role of the EphB4 receptor in normal and diseased states is of great therapeutic importance.
Source	Rabbit
Specificity	The EPHB4 Antibody has the ability to recognize total EPHB4 levels that occur naturally within the body. By using this antibody, it is possible to gain insights into the expression and activity of EPHB4.
Species Reactivity	Human;Mouse
Predictive reaction species	Xenopus;Rabbit;Pig;Dog;Sheep;Bovine;Horse
Antigen	EPHB4
Application	The dilution ratios for WB, IHC, and ELISA(peptide) are as follows: WB is diluted at a range of 1:500 to 1:2000, IHC is diluted at a range of 1:50 to 1:200, and ELISA (peptide) is diluted at a range of 1:20000 to 1:40000. Please note that this content has been rearranged for clarification purposes but retains the original information.
Immunogen	A synthesized peptide derived from human EPHB4.
MW	108 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was subjected to peptide affinity chromatography to achieve purification. This process ensured that impurities were eliminated, resulting in a highly purified antiserum product.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the integrity of the product, it is essential to store it at a temperature of -20 °C for a duration of one year. It is advisable to prevent any instances of repeated freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis.
Tissue specificity	Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix.
Posttranslational modification	Phosphorylated; autophosphorylation is stimulated by EFNB2.
Celluar localization	Cytosol;Extracellular region or secreted;Plasma Membrane;
UniPort	P54760