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Rabbit Anti-EIF3F Polyclonal Antibody#abs141132

Important: please note that the price mentioned above is for reference purposes only. For accurate and detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Western blot analysis of extracts from Jurkat cells and COLO205 cells, using EIF3F antibody. This...

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Description

Catalog-specification	Delivery time	USD price
abs141132-100ug	1-2 Weeks	301.0
abs141132-50ug	1-2 Weeks	201.0

Important: please note that the price mentioned above is for reference purposes only. For accurate and detailed pricing information, we kindly request you to get in touch with our seller, Vecent.

Overview
catalog	abs141132
Other names	eIF3f, also known as eIF-3-epsilon or eIF3 epsilon, is a deubiquitinating enzyme. It is sometimes referred to as eIF3 p47 or eIF3 p47 subunit. In the past, it was called EIF3S5. This protein is a subunit of eukaryotic translation initiation factor 3 (eIF3), specifically subunit 5. It has a molecular weight of 47kDa. Its official symbol is EIF3F_HUMAN. eIF3f plays a crucial role in the initiation of protein synthesis, as a part of the eIF3 complex. It is involved in the removal of ubiquitin molecules from target proteins. Understanding the function and mechanism of eIF3f is important in unraveling the complexities of translation initiation in eukaryotic cells.
Source	Rabbit
Specificity	The total EIF3F levels are detected by the EIF3F Antibody, which specifically recognizes the endogenous expression of EIF3F protein. To ensure accurate detection, the antibody has been designed to target all isoforms and variants of EIF3F.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Bovine;Horse;
Antigen	EIF3F
Application	The recommended dilutions for WB are 1:500~1:1000, for IHC they are 1:50~1:200, for IF/ICC they are 1:100-1:500, and for ELISA (peptide) they are 1:20000-1:40000.
Immunogen	A synthesized peptide.
MW	38 KD
Properties
Concentration	1mg/ml
purification	The purification of the antiserum involved the utilization of SulfoLink™ Coupling Resin for peptide affinity chromatography. This process helped in isolating and extracting the necessary components of the antiserum efficiently.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).
Posttranslational modification	Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11.
Celluar localization	Cytosol;
UniPort	O00303