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Rabbit Anti-Dnmt1 Polyclonal Antibody#abs136888

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Description

Catalog-specification	Delivery time	USD price
abs136888-100ug	1-2 Weeks	301.0
abs136888-50ug	1-2 Weeks	201.0

Overview
catalog	abs136888
Description	The role of DNA (cytosine-5-)-methyltransferase 1 in the establishment and regulation of tissue-specific patterns of methylated cytosine residues is crucial. Abnormal methylation patterns have been linked to specific human tumors and developmental abnormalities. Interestingly, this gene exhibits two transcript variants, which encode different isoforms. These isoforms likely contribute to the diverse functions of DNA (cytosine-5-)-methyltransferase 1. It is intriguing to explore how these isoforms contribute to the establishment and maintenance of methylated cytosine patterns in various tissues. Furthermore, understanding the implications of aberrant methylation patterns driven by DNA (cytosine-5-)-methyltransferase 1 could unveil novel therapeutic targets for human tumors and developmental disorders. Overall, this research sheds light on the intricate mechanisms involved in the regulation of DNA methylation and its potential impact on human health.
Other names	There are several different names for the same gene: ADCADN, AIM, CXXC finger protein 9, CXXC-type zinc finger protein 9, CXXC9, DNA (cytosine 5 ) methyltransferase 1, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase 1, DNA methyltransferase HsaI, DNA methyltransferase M.HsaI, DNA MTase, DNA MTase HsaI, DNMT 1, DNMT, Dnmt1, DNMT1_HUMAN, Dnmt1o, FLJ16293, HSN1E, M.HsaI, MCMT, Met1, MGC104992, mMmul, and MommeD2. Despite all these names, they all refer to the same gene.
Source	Rabbit
Specificity	The endogenous levels of total Dnmt1 can be effectively detected using the Dnmt1 Antibody.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Horse
Antigen	Dnmt1
Application	The recommended dilutions for using the protein in various applications are as follows: 1:500 to 1:2000 for Western blotting, 1:50 to 1:200 for immunohistochemistry, 1:100 to 400 for immunofluorescence, and 1:20000 to 1:40000 for ELISA (peptide). These dilutions are suggested as a starting point and may need to be optimized for each experiment. It is important to use the proper dilution to obtain accurate and reliable results.
Immunogen	A synthesized peptide derived from human Dnmt1.
MW	183kDa
Properties
Concentration	1mg/ml
purification	We utilized SulfoLink™ Coupling Resin in peptide affinity chromatography to purify the antiserum. Let me provide you with a similar content by rearranging the information from the original text.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). Promotes tumor growth (PubMed:24623306).
Tissue specificity	Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
Posttranslational modification	Sumoylated; sumoylation increases activity.Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G2/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity.Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 therebye increasing DNMT1 stability.Methylation at Lys-142 by SETD7 promotes DNMT1 proteasomal degradation.Ubiquitinated by UHRF1; interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome.
Celluar localization	Nucleus;
UniPort	P26358