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Rabbit Anti-Cullin2 Polyclonal Antibody#abs130078

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Description

Catalog-specification	Delivery time	USD price
abs130078-100ug	1-2 Weeks	301.0
abs130078-50ug	1-2 Weeks	201.0

Overview
catalog	abs130078
Description	The core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes is CUL2. These complexes play a crucial role in mediating the ubiquitination of target proteins. CUL2 acts as a rigid scaffold within the complex and may contribute to the catalytic activity by positioning the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase function of the complex relies on the neddylation process of the cullin subunit. This function is hindered when the deneddylated cullin subunit associates with TIP120A/CAND1. The ECS complex's functional specificity is determined by its substrate recognition component. ECS(VHL) specifically targets the hypoxia-inducible factor (HIF) for ubiquitination.
Other names	Cullin 2 is known as Cul2, CUL-2, CUL2, or CUL2_HUMAN. It is a homolog of cullin, specifically cullin 2. Another name for it is Cullin-2 or MGC131970.
Source	Rabbit
Specificity	The ability to detect endogenous levels of total Cullin 2 is a significant characteristic of the Cullin 2 antibody. This antibody allows researchers to accurately measure the presence of Cullin 2 within biological samples. By recognizing and binding to Cullin 2, the antibody aids in the identification and quantification of this protein. This capability is crucial for studying the roles and functions of Cullin 2 in various biological processes. With the Cullin 2 antibody, researchers can gather valuable insights into the expression and regulation of Cullin 2, contributing to a deeper understanding of its involvement in cellular pathways and disease mechanisms.
Species Reactivity	Human;Mouse
Predictive reaction species	Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;
Antigen	Cullin2
Application	The recommended dilutions for this antibody are 1:500 to 1:3000 for Western blotting, 1:50 to 1:200 for immunohistochemistry, 1:100 to 1:500 for immunofluorescence or immunocytochemistry, and 1:20000 to 1:40000 for ELISA using peptide. It is important to carefully optimize the dilution for each individual experiment to ensure the best possible results. So, be sure to leverage the dilution recommendations provided and consider optimizing the dilution to better suit your experimental needs.
Immunogen	A synthesized peptide derived from human Cullin 2.
MW	82kDa,84kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
Posttranslational modification	Neddylated; which enhances the ubiquitination activity of ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes. CBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity).
Celluar localization	Cytosol;Nucleus;
UniPort	Q13617