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Rabbit Anti-CBLB Polyclonal Antibody#abs136273

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Description

Catalog-specification	Delivery time	USD price
abs136273-100ug	1-2 Weeks	301.0
abs136273-50ug	1-2 Weeks	201.0

Overview
catalog	abs136273
Description	Cbl proteins, including c-Cbl, Cbl-b, and Cbl-c, belong to the Casitas B lineage lymphoma family of single subunit RING finger protein-ubiquitin E3 ligases. The N-terminal tyrosine kinase-binding (TKB) domain of Cbl proteins enables them to interact with phosphorylated tyrosine residues on Cbl substrates, while the proline-rich domains, C-terminal to the RING finger, mediate interactions with SH3 domain-containing proteins. As adaptor proteins, Cbl proteins target specific substrates for ubiquitination and degradation, and they also play a role in receptor tyrosine kinase internalization and activation of signaling pathways. In particular, Cbl-b is a well-studied E3 ubiquitin ligase that downregulates TCR expression during antigen recognition and acts as a negative regulator of CD28 signaling cascade by ubiquitinating the p85 regulatory subunit of PI3K. Cbl-b mRNA and protein are upregulated in T cells following calcium mobilization and calcineurin activation, and its deficiency can lead to resistance to anergy induction due to defects in the ubiquitination of PLCÎ³1 and PKCÎ¸.
Other names	B-lineage lymphoma proto-oncogene b, also known as Cas Br M (murine) ecotropic retroviral transforming sequence b, is a protein encoded by the CBLB gene. It belongs to the Cbl family of E3 ubiquitin protein ligases and is involved in signal transduction pathways. CBLB has a RING finger domain and a SH3 binding domain. Its primary function is to regulate the degradation of target proteins through the ubiquitin-proteasome system. CBLB plays a role in various cellular processes, including cell growth, differentiation, and immune response. It is expressed in various tissues and is associated with the development of certain cancers. The exact mechanisms by which CBLB exerts its biological effects are still being studied.
Source	Rabbit
Specificity	CBLB Antibody detects endogenous levels of total CBLB.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Horse
Antigen	CBLB
Application	WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human CBLB.
MW	109kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLinkâ„¢ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 Â°C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 Â°C.Stable for 12 months from date of receipt.
Target
Background	E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.
Tissue specificity	Expressed in placenta, heart, lung, kidney, spleen, ovary and testis, as well as fetal brain and liver and hematopoietic cell lines, but not in adult brain, liver, pancreas, salivary gland, or skeletal muscle. Present in lymphocytes (at protein level).
Posttranslational modification	Phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation.Auto-ubiquitinated upon EGF-mediated cell activation or upon T-cell costimulation by CD28; which promotes proteasomal degradation.
Celluar localization	Cytosol;Nucleus;Plasma Membrane;
UniPort	Q13191