Rabbit Anti-CASP3 Polyclonal Antibody#abs135540
Please note that the price provided is for your reference only. For more detailed pricing information, we suggest contacting our sales representative, Vecent. 제공된 가격은 참고용이며, 보다 자세한 가격 정보를 원하시면 저희 판매 대표인 Vecent에게 문의해주시기 바랍니다. This product is for research use only, not for use in diagnostic...
Description
| Catalog-specification | Delivery time | USD price |
abs135540-50ug | 1-2 Weeks | 201.0 |
abs135540-100ug | 1-2 Weeks | 301.0 |
Please note that the price provided is for your reference only. For more detailed pricing information, we suggest contacting our sales representative, Vecent. 제공된 가격은 참고용이며, 보다 자세한 가격 정보를 원하시면 저희 판매 대표인 Vecent에게 문의해주시기 바랍니다.
| Overview | |
catalog | abs135540 |
Description | Caspase-3, also known as CPP-32, Apoptain, Yama, or SCA-1, has a vital role in triggering apoptosis by cleaving several important proteins including nuclear enzyme poly(ADP-ribose) polymerase (PARP). Its activation requires the conversion of its inactive form into activated p17 and p12 fragments through proteolytic processing. The cleavage of caspase-3 is specifically facilitated by the presence of aspartic acid at the P1 position. |
| Other names | A830040C14Rik is a gene that encodes for a protein called Apopain, also known as CASP-3 or Caspase 3. CASP3_HUMAN is the human version of this protein. It is an enzyme that plays a crucial role in apoptosis, which is a programmed cell death process. The protein has several aliases including Casp3a, Caspase 3, Caspase 3, apoptosis-related cysteine peptidase, Caspase 3, apoptosis-related cysteine protease, Caspase 3, apoptosis-related cysteine protease a, Caspase-3 subunit p12, CC3, CPP-32, CPP32, CPP32B, Cysteine protease CPP32, EC 3.4.22.56, LICE, mldy, OTTHUMP00000165052, OTTHUMP00000165053, OTTHUMP00000165054, PARP cleavage protease, Procaspase3, protein Yama, SCA 1, SCA-1, SREBP cleavage activity 1, and Yama. Please note that the rearranged content might not maintain the same level of accuracy as the original text. |
| Source | Rabbit |
| Specificity | The total CASP3 levels are detected by the CASP3 Antibody, enabling the identification of endogenous levels of CASP3. |
| Species Reactivity | Human;Mouse;Rat |
| Antigen | CASP3 |
| Application | IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA (peptide) 1:20000-1:40000 - these are the recommended dilutions for various immunological assays. For Western Blot (WB), the suggested dilution ranges from 1:500 to 1:2000. Immunohistochemistry (IHC) should be performed at dilutions ranging from 1:50 to 1:200. For Immunofluorescence/Immunocytochemistry (IF/ICC), dilution ranging from 1:100 to 1:500 is recommended. Lastly, in Enzyme-Linked Immunosorbent Assays (ELISA) using peptides, the optimal dilution is suggested to be within the range of 1:20000 to 1:40000. These dilutions ensure accurate and reliable results in each respective assay. |
| Immunogen | A synthesized peptide derived from human CASP3. |
| MW | 32kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | Using SulfoLink™ Coupling Resin, the antiserum underwent peptide affinity chromatography for purification. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. |
| Tissue specificity | Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. |
| Posttranslational modification | Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. |
| Celluar localization | Cytosol;Nucleus(cleaved);Plasma Membrane; |
| UniPort | P42574 |
This product is for research use only, not for use in diagnostic prodecures or in human.
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