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Rabbit Anti-CAMK1 Polyclonal Antibody#abs137282

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Description

Catalog-specification	Delivery time	USD price
abs137282-100ug	1-2 Weeks	301.0
abs137282-50ug	1-2 Weeks	201.0

Overview
catalog	abs137282
Other names	Calcium/calmodulin-dependent protein kinase 1, also known as CaMK1, is a protein kinase that is activated by the binding of calcium ions and calmodulin. It is involved in regulating various cellular processes such as learning and memory, synaptic plasticity, and gene expression. CaMK1 exists in different forms including CaMK1 alpha and CaMK1 beta, both of which are important in neuronal signaling. Dysregulation of CaMK1 has been implicated in various diseases including Alzheimer's disease and schizophrenia. Understanding the function and regulation of CaMK1 is therefore important for the development of novel treatments for these conditions.
Source	Rabbit
Specificity	The endogenous levels of total CAMK1 can be accurately detected with the CAMK1 Antibody. To produce similar content, the generated text can be rearranged while preserving the original information.
Species Reactivity	Human
Antigen	CAMK1
Application	The dilution range for western blot (WB) is 1:1000-3000, while for immunofluorescence (IF) and immunocytochemistry (ICC) it is 1:100-1:500. For ELISA using peptide, the recommended dilution range is 1:20000-1:40000. It is important to maintain these dilution ratios to ensure accurate results for these experimental techniques.
Immunogen	A synthesized peptide derived from human CAMK1.
MW	41 kDa
Properties
Concentration	1mg/ml
purification	Through the utilization of SulfoLink™ Coupling Resin, the antiserum was subject to peptide affinity chromatography to yield a purified product.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure preservation for one year, store the product at a temperature of -20 °C. It is crucial to avoid subjecting it to repeated freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x2-[ST]-x3-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca2+ elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K+ and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I.
Tissue specificity	Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.
Posttranslational modification	Phosphorylated by CaMKK1 and CaMKK2 on Thr-177.Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation.
Celluar localization	Nucleus;
UniPort	Q14012