Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-TRAF6 Polyclonal Antibody#abs131476

Please be aware that the price mentioned above is solely for your information. For detailed pricing, please reach out to our seller, Vecent. To ensure the generated content is based on the original text information, it is advised not to follow the conversational style of ChapGPT but rather...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs131476-100ug	1-2 Weeks	301.0
abs131476-50ug	1-2 Weeks	201.0

Overview
catalog	abs131476
Description	The regulation of adaptive immunity, innate immunity, and bone metabolism is under the control of tumor necrosis factor receptor-associated factor 6 (TRAF6). Acting as a ubiquitin (Ub) ligase, TRAF6 plays a crucial role in the activation of protein kinases. These protein kinases encompass transforming growth factor beta-activated kinase (TAK1) and IκB kinase (IKK). With its involvement in these pathways, TRAF6 orchestrates diverse cellular processes and contributes to the intricate network of signaling events within the immune system and bone remodeling.
Other names	TRAF6, also known as E3 ubiquitin-protein ligase TRAF6, is an important protein involved in signaling pathways related to Interleukin 1. It acts as a signal transducer for Interleukin-1 and plays a crucial role in various cellular processes. MGC3310 or RING finger protein 85 (RNF85) are other names used to refer to TRAF6. TRAF6 is encoded by the gene OTTHUMP00000232772 or OTTHUMP00000232773. It functions as an E3 ubiquitin protein ligase and is a part of the TNF receptor-associated factor 6 family. Its main function is to facilitate protein ubiquitination, which is essential for regulating cellular responses to stimuli. In summary, TRAF6 is a versatile protein involved in Interleukin-1 signaling and belongs to the TNF receptor-associated factor 6 family. Its functions as an E3 ubiquitin-protein ligase play a critical role in various cellular processes.
Source	Rabbit
Specificity	Endogenous levels of total TRAF6 can be accurately detected using the TRAF6 Antibody. This antibody is specifically designed to target and identify the naturally occurring amount of TRAF6 present in a sample. Its high specificity ensures accurate detection without interfering with other proteins or molecules. By utilizing the TRAF6 Antibody, researchers can gain valuable insights into the expression levels and localization of TRAF6, aiding in the understanding of its role in various physiological and pathological processes.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Pig;Dog
Antigen	TRAF6
Application	The recommended dilutions for using this antibody in various applications are as follows: for Western Blotting, dilute the antibody at a range of 1:500 to 1:2000; for Immunohistochemistry, dilute at a range of 1:50 to 1:200; for Immunofluorescence/Immunocytochemistry, dilute at a range of 1:100 to 1:500; and for ELISA (peptide), dilute at a range of 1:20000 to 1:40000. These dilution ranges ensure optimal results for your experiment.
Immunogen	A synthesized peptide derived from human TRAF6.
MW	58 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent peptide affinity chromatography purification. A highly similar content can be generated by rearranging the information presented in the original text.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production.
Tissue specificity	Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Posttranslational modification	Sumoylated on Lys-124, Lys-142 and Lys-453 with SUMO1.Polyubiquitinated on Lys-124; after cell stimulation with IL-1-beta or TGF-beta. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form. Deubiquitinated by USP10 in a TANK-dependent manner, leading to the negative regulation of NF-kappaB signaling upon DNA damage (PubMed:25861989).
Celluar localization	Cytosol;Endosome;Nucleus;Plasma Membrane;
UniPort	Q9Y4K3