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Rabbit Anti-BIRC2 Polyclonal Antibody#abs135687

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Description

Catalog-specification	Delivery time	USD price
abs135687-100ug	1-2 Weeks	301.0
abs135687-50ug	1-2 Weeks	201.0

Overview
catalog	abs135687
Description	IAP proteins, known as inhibitors of apoptosis protein, form a family of apoptosis inhibitors that have a conserved 70 amino acid BIR domain. This group of proteins, including c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP, is evolutionarily conserved among humans (1,2). Their presence in cancer cell lines and tumors, particularly the overexpression of survivin and livin, indicates a significant role in cancer progression (3-5). These IAP proteins mainly function by directly interacting with caspases, such as caspase-3, caspase-7, and caspase-9, to inhibit their activity (5,6). Additionally, the binding of IAP family members to the mitochondrial protein Smac prevents their interaction with caspase-9, ultimately allowing the processing and activation of this caspase (2). Hence, IAP proteins have intricate mechanisms to regulate and suppress apoptosis, making them an important focus of cancer research.
Other names	Baculoviral IAP repeat-containing protein 2, also known as API1 or Apoptosis inhibitor 1, is a protein that plays a critical role in regulating cell death processes. It is encoded by the BIRC2 gene and has been found to be involved in various signaling pathways. The function of BIRC2 is primarily associated with inhibiting apoptosis, which is a programmed cell death mechanism. By preventing apoptosis, BIRC2 helps to maintain cellular homeostasis and promote cell survival. It achieves this by binding to and inhibiting caspases, which are proteases responsible for initiating apoptosis. Additionally, BIRC2 can also regulate other cellular processes such as inflammation and immune response through its interactions with various proteins. BIRC2 is a member of the inhibitor of apoptosis (IAP) protein family, which is characterized by the presence of baculoviral IAP repeats (BIRs) in their protein structure. These BIR domains allow BIRC2 to interact with a range of proteins involved in cell death regulation. Furthermore, BIRC2 contains a RING finger domain, which is involved in protein-protein interactions and ubiquitin ligase activity. In addition to its role in inhibiting apoptosis, BIRC2 has been implicated in cancer development and progression. It is often overexpressed in various types of tumors and its upregulation has been associated with increased cell survival and treatment resistance. As a result, targeting BIRC2 has emerged as a potential therapeutic strategy for cancer treatment. In summary, BIRC2 is a crucial protein involved in the regulation of cell death processes. Its inhibition of apoptosis and interactions with other signaling molecules make it a key player in maintaining cellular homeostasis and promoting cell survival. Understanding the functions of BIRC2 provides insights into the development of potential therapies for diseases such as cancer.
Source	Rabbit
Specificity	The BIRC2 Antibody is capable of detecting total endogenous levels of BIRC2 in various samples. This antibody specifically targets the BIRC2 protein and is a reliable tool for researchers conducting experiments on the detection of this protein in different tissue samples. By analyzing the total amount of BIRC2 present, researchers can gain a better understanding of the role this protein plays in the body and how it may be involved in different cellular processes. Overall, the BIRC2 Antibody is a valuable tool for any researcher that needs to detect and measure the levels of BIRC2 in various samples.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Dog
Antigen	BIRC2
Application	The recommended dilution for WB is between 1:500 to 1:2000, for IHC it is between 1:50 to 1:200, and for IF it is between 1:100 to 400. For ELISA (peptide), it is suggested to dilute the solution in a range of 1:20,000 to 1:40,000. This dilution range is suitable for accurate and reliable results. It is important to note that these dilution ratios may vary depending on the specific experiment and sample types. When using these techniques, it is essential to follow the recommended protocols and guidelines provided by the manufacturer to ensure optimal results.
Immunogen	A synthesized peptide derived from human BIRC2.
MW	70kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF2, DIABLO/SMAC, MAP3K14/NIK, MAP3K5/ASK1, IKBKG/NEMO, IKBKE and MXD1/MAD1. Can also function as an E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Can stimulate the transcriptional activity of E2F1. Plays a role in the modulation of the cell cycle.
Tissue specificity	Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.
Posttranslational modification	Auto-ubiquitinated and degraded by the proteasome in apoptotic cells.
Celluar localization	Cytosol;Nucleus;Plasma Membrane;
UniPort	Q13490