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Rabbit Anti-BID Polyclonal Antibody#abs135536

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Description

Catalog-specification	Delivery time	USD price
abs135536-50ug	1-2 Weeks	201.0
abs135536-100ug	1-2 Weeks	301.0

Overview
catalog	abs135536
Description	BID, a member of the Bcl-2/Bcl-xL family, is a death agonist protein that contains the BH3 domain. Originally, it localizes in the cytosolic fraction of cells as an inactive precursor. However, upon cleavage by caspase-8 in the Fas signaling pathway, BID is activated and translocates to mitochondria, inducing mitochondrial damage and cytochrome c release. As a result, BID plays a significant role in transmitting apoptotic signals from the cell surface to mitochondria. Nonetheless, the exact mechanism by which cleaved BID translocates and cytochrome c is released remains unclear.
Other names	BID, also known as apoptotic death agonist or BH3 interacting domain death agonist, is a protein involved in the apoptotic pathway. It has various isoforms, including BID isoform ES(1b), BID isoform L(2), and BID isoform Si6. Other related terms for BID include BH3 interacting domain death agonist p11, BH3 interacting domain death agonist p13, BH3 interacting domain death agonist p15, and BH3-interacting domain death agonist p11. Additionally, BID is also referred to as p11 BID, p13 BID, p15 BID, or p22 BID. Another name for BID is FP497. It is encoded by the human BID coding sequence and is also known as MGC15319 or MGC42355. Along with its association with apoptosis, BID is sometimes linked to desmocollin type 4.
Source	Rabbit
Specificity	Total BID levels can be detected by the BID Antibody, which targets endogenous BID proteins.
Species Reactivity	Human;Mouse;Rat
Antigen	BID
Application	The recommended dilution range for WB is from 1:500 to 1:2000, while for IHC it is 1:50 to 1:200. For ELISA using peptide, it is suggested to use a dilution of 1:20000 to 1:40000. These dilution ranges are based on optimal results from previous tests and are intended to ensure accurate and reliable detection of the target protein. It is important to follow the instructions carefully and adjust the dilution ratio as needed to achieve the best possible results for your specific application.
Immunogen	A synthesized peptide derived from human BID.
MW	22kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
Tissue specificity	Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
Posttranslational modification	TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.
Celluar localization	Cytosol;Extracellular region or secreted;Mitochondrion;
UniPort	P55957