Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-BCL2L1 Polyclonal Antibody#abs135535

Please note that the price mentioned above is only for your reference. In order to obtain detailed information on pricing, kindly get in touch with our seller Vecent. Western blot analysis of extracts of Hela celllines, using BCL2L1 antibody.The lane on the left is treated with the...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs135535-100ug	1-2 Weeks	301.0
abs135535-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. In order to obtain detailed information on pricing, kindly get in touch with our seller Vecent.

Overview
catalog	abs135535
Description	This gene encodes a protein that belongs to the BCL-2 protein family, which is known for its anti- and pro-apoptotic properties. It has been shown that this protein can form hetero- or homodimers and is located at the outer mitochondrial membrane. Its main function involves the regulation of outer mitochondrial membrane channel (VDAC) opening that controls the mitochondrial membrane potential. Regulating VDAC opening can significantly affect the production of reactive oxygen species and release of cytochrome C, both of which are potent inducers of cell apoptosis. There are two alternatively spliced transcript variants of this gene, and they encode distinct isoforms. The longer isoform acts as an inhibitor of apoptosis, while the shorter form acts as an activator of apoptosis. These isoforms perform different functions, which can significantly affect cellular activities and processes. Overall, this protein plays a crucial role in regulating the balance between cell survival and cell death, which is essential for maintaining tissue homeostasis and preventing the onset of various diseases.
Other names	Bcl-X is a crucial regulator of apoptosis, also known as programmed cell death. It belongs to the Bcl-2 family and is involved in the regulation of cell survival and apoptosis. The protein, encoded by the Bcl2l1 gene, is alternatively spliced into two isoforms: Bcl-XL and Bcl-XS. Bcl-XL, which stands for Bcl-2 like 1 protein, is the long isoform that promotes cell survival by inhibiting apoptosis. On the other hand, the short isoform, Bcl-XS, promotes apoptosis. Bcl-X plays a critical role in maintaining the balance between cell survival and programmed cell death. It functions by regulating the permeability of the mitochondrial membrane and preventing the release of cytochrome c, which is crucial for initiating cell death signaling pathways. By inhibiting apoptosis, Bcl-X aids in the survival of cells, especially in response to various stressors and insults. The Bcl-X protein is known for its anti-apoptotic activity and has been implicated in numerous cellular processes, including embryonic development, tissue homeostasis, and immune response. Its dysregulation has been associated with various diseases, particularly cancer, where increased expression of Bcl-XL can contribute to the survival and resistance of cancer cells against chemotherapy and radiation therapy. Overall, Bcl-X is a pivotal component in the intricate network of apoptotic regulation. Its ability to modulate the delicate balance between cell survival and death makes it an attractive target for therapeutic interventions aimed at manipulating apoptotic pathways in various diseases.
Source	Rabbit
Specificity	The endogenous levels of total BCL2L1 can be accurately detected by using the BCL2L1 Antibody.
Species Reactivity	Human;Mouse;Rat
Antigen	BCL2L1
Application	To use the antibody for Western blot, dilute it at a ratio of 1:500 to 1:2000. If using it for immunohistochemistry, dilute at a ratio of 1:50 to 1:200. For immunofluorescence and immunocytochemistry, the recommended dilution is 1:100 to 1:500. If using the antibody for ELISA with a peptide, dilute it at a ratio of 1:20000 to 1:40000. By adjusting the dilution rate, the antibody can be optimized for various applications.
Immunogen	A synthesized peptide derived from human BCL2L1.
MW	26kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.
Tissue specificity	Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
Posttranslational modification	Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis.
Celluar localization	Cytoskeleton;Cytosol;Endoplasmic reticulum;Mitochondrion;Nucleus;
UniPort	Q07817