Phospho-AurB (Thr232) Rabbit Polyclonal Antibody#abs131100

abs131100-50ug

1-2 Weeks

201

abs131100-100ug

1-2 Weeks

301

Description

Kinases and phosphatases are responsible for regulating chromosomal segregation in both mitosis and meiosis. During chromosome movement and segregation, the Aurora kinases form associations with microtubules.

Other names

AURKB, also known as Aurora kinase B, is a protein involved in cell division and regulation of the cell cycle. It is associated with midbody formation and is part of the Aurora/IPL1 family of kinases. AURKB plays a crucial role in maintaining genomic stability during mitosis. It functions by phosphorylating various substrates involved in mitotic progression. Its activity is tightly regulated and it is important for proper chromosome segregation and cytokinesis. AURKB is also associated with other proteins such as PPP1R48, which acts as a regulatory subunit for protein phosphatase 1. This kinase is classified as a serine/threonine kinase due to its ability to phosphorylate serine and threonine residues on target proteins. Its role in cell division and its involvement in various cellular processes make AURKB a key player in maintaining cell homeostasis.

Source

Rabbit

Specificity

The Phospho-AurB (Thr232) Antibody specifically recognizes the presence of phosphorylated Threonine 232 in endogenous levels of AurB. This antibody does not detect AurB unless it is phosphorylated at Threonine 232. To reiterate, the antibody is designed to specifically target and detect AurB when it is phosphorylated at this particular site.

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Horse;Bovine;

Application

We recommend using this antibody at dilutions of WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, and ELISA(peptide) 1:20000-1:40000 to achieve optimal results. These dilutions have been empirically determined to ensure reliable and reproducible results. Our antibody has been rigorously tested in various applications to ensure maximum sensitivity and specificity. Dilution optimization may be necessary depending on the sample type and experimental conditions. Please refer to the product datasheet for additional details.

Human AurB-derived synthesized peptide around the phosphorylation site of Threonine 232 is readily available. I can provide you with a peptide that closely resembles the original sequence. Let me know if you require any further assistance.

Properties

1mg/ml

Purification

The purification process for the antibody involved the use of affinity columns for both phospho- and non-phospho-peptides. Purified rabbit serum was utilized in the production of this highly effective and specific antibody, which was then purified through a series of sequential chromatography steps. This ensured that the final product was of the highest quality and specifically targeted the intended antigen.

Clonality

Polyclonal Antibody

To ensure optimal storage, it is recommended to keep the product at a temperature of -20 °C for up to one year. It is important to avoid exposing the product to repeated freeze/thaw cycles, as this can potentially compromise its integrity and efficacy. Take the necessary precautions to maintain the product's stability and ensure its effectiveness over time.

Storage buffer

This product is Rabbit IgG, which comes in a phosphate-buffered saline solution with pH 7.4, 150mM NaCl, and 0.02% sodium azide. It also contains 50% glycerol and should be stored at -20 °C. This solution is stable for up to 12 months from the date of receipt.

Target

Background

 Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (PubMed:22422861, PubMed:24814515). AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, GSG2/Haspin, and histone H3. A positive feedback loop involving GSG2 and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between GSG2 and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGO1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes.

Tissue specificity

High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.

Celluar localization

Cytoskeleton;Cytosol;Nucleus;

Q96GD4