Rabbit Anti-Histone H3K27me2 Polyclonal Antibody#abs136460
Please note that the price mentioned here is just for your reference and may not be accurate. For the actual price details, kindly get in touch with our sales representative, Vecent. We would be happy to provide you with all the necessary information. Western blot analysis of extracts of HeLa...
Description
| Catalog-specification | Delivery time | USD price |
abs136460-100ug | 1-2 Weeks | 301.0 |
abs136460-50ug | 1-2 Weeks | 201.0 |
Please note that the price mentioned here is just for your reference and may not be accurate. For the actual price details, kindly get in touch with our sales representative, Vecent. We would be happy to provide you with all the necessary information.
| Overview | |
catalog | abs136460 |
Description | The regulation of gene expression in eukaryotes heavily relies on the modulation of chromatin structure. The fundamental unit of chromatin, known as the nucleosome, consists of DNA wound around eight core histone proteins. These core histones, which comprise two copies each of H2A, H2B, H3, and H4, can undergo various post-translational modifications such as acetylation, phosphorylation, methylation, and ubiquitination. These modifications are influenced by different stimuli and have a direct impact on the accessibility of chromatin to transcription factors, thereby affecting gene expression. |
| Other names | The HIST1 cluster, also known as the H3 histone family, consists of several members such as H3.3A, H3E, H3.1, H3F3, and H3FF. These members play important roles in gene regulation and DNA packaging. Histone H3.1, also referred to as H3.1t or Histone H3/o, is a variant of the H3 histone family. It is encoded by the FLJ92264 gene and is involved in maintaining the structure and stability of chromatin. Other members of the H3 histone family include H3 histone family members B, C, D, F, H, I, J, K, L, and T. These members are closely related and share similar functions in the nucleus. The H3 histone family plays a crucial role in gene expression and DNA replication by organizing the DNA into a compact and stable structure. It helps regulate the access of transcription factors and other proteins to the DNA, thus influencing gene activity. The histone cluster 1, H3 histone family, consists of different variants such as H3A, H3B, H3C, H3D, H3E, H3F, H3G, H3H, H3I, and H3J. These variants are encoded by specific genes within the histone gene cluster 1. In addition to their role in gene regulation, the histone cluster 1, H3 histone family members A, B, C, D, E, F, G, H, I, and J have been associated with various cellular processes including DNA repair, cell division, and development. Overall, the H3 histone family and its variants are essential components of chromatin and have significant implications in numerous biological processes. Their intricate regulation and interactions contribute to the complexity and diversity of gene expression in multicellular organisms. |
| Source | Rabbit |
| Specificity | Histone H3K27me2 Antibody detects endogenous levels of total Histone H3K27me2. |
| Species Reactivity | Human;Mouse;Rat |
| Antigen | Histone H3K27me2 |
| Application | WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50-1:200, IP 1:50-1:200, ELISA(peptide) 1:20000-1:40000 |
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3. |
| MW | 15kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin . |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Tissue specificity | Expressed in testicular cells. |
| Posttranslational modification | Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability (By similarity).Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters (By similarity).Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication (By similarity).Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MAP3K20 isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin (By similarity).Ubiquitinated.Lysine deamination at Lys-5 (H3K4all) to form allysine is mediated by LOXL2. Allysine formation by LOXL2 only takes place on H3K4me3 and results in gene repression (By similarity). |
| Celluar localization | Extracellular region or secreted;Nucleus; |
| UniPort | Q16695 |
Western blot analysis of extracts of HeLa cellline and H3 protein expressed in E.coli., usingH3K27me2antibody|Dot-blot analysis of all sorts of methylation peptides using H3K27me2 antibody|Immunofluorescence analysis of 293T cell using H3K27me2 antibody. Blue: DAPI for nuclear staining.
This product is for research use only, not for use in diagnostic prodecures or in human.
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