Rabbit Anti-Histone H3K27me1 Polyclonal Antibody#abs136459
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Description
| Catalog-specification | Delivery time | USD price |
abs136459-100ug | 1-2 Weeks | 301.0 |
abs136459-50ug | 1-2 Weeks | 201.0 |
Please note that the price mentioned above is for reference purposes only, and for more accurate pricing details, kindly get in touch with our sales representative Vecent. It is important to understand that the original content can be used as a basis for generating similar content, while ensuring that the information presented is in line with the original text. Therefore, it is recommendable to use a language model that can generate text that is significantly different from the original content.
| Overview | |
catalog | abs136459 |
Description | Regulation of gene expression is tightly linked to chromatin structure that is modulated via a variety of mechanisms in eukaryotes. The nucleosome, comprising of DNA wrapped around histones, is a fundamental component of chromatin that undergoes various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications are triggered by a diverse range of stimuli, and they directly impact the accessibility of chromatin to different transcription factors, regulating gene expression. In most species, histone H2B is predominantly acetylated at Lys5, 12, 15, and 20, while H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Additionally, phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is essential for chromosome condensation during mitosis and meiosis. Highly conserved phosphorylation at Thr3 of histone H3 is catalyzed by the kinase haspin, and its detection via phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase. In some organisms, acetylation of H3 at Lys9 is the primary modulator of histone deposition and chromatin assembly. |
| Other names | Histone H3 is a vital protein involved in DNA packaging and gene regulation. It belongs to the H3 histone family and is encoded by various genes, such as HIST1 cluster, H3 histone family, member A (H3.3A) and H3E. Other members of this family include H3.1, H3F3, H3FF, H3FJ, and H3FL. These different variants of histone H3 play significant roles in chromatin structure and function. Histone H3.1 and H3.3 are two major subtypes, with H3.1 being the canonical form found in most cells and H3.3 being a replication-independent variant involved in dynamic chromatin remodeling. They share high sequence similarity but differ in their deposition mechanisms and functions. The histone H3 gene cluster consists of multiple members, including H3 histone family members B, C, D, F, H, I, J, K, L, and T. These genes are located closely together and are involved in producing various isoforms of histone H3. Each member plays a distinct role in regulating gene expression and ensuring proper DNA packaging. In addition to the canonical isoforms, there are also alternative variants of histone H3, such as H3.1t and H3/o. These variants may have specific functions in certain cell types or developmental stages. Although there are multiple names and designations for histone H3, they all refer to the same protein that forms the core of nucleosomes and contributes to chromatin structure. The intricate regulation and variety of histone H3 isoforms highlight the complexity and importance of epigenetic mechanisms in gene expression and cellular processes. |
| Source | Rabbit |
| Specificity | Histone H3K27me1 Antibody detects endogenous levels of total Histone H3K27me1. |
| Species Reactivity | Human;Mouse |
| Antigen | Histone H3K27me1 |
| Application | WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50-1:200, ELISA(peptide) 1:20000-1:40000 |
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3. |
| MW | 15kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin . |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Tissue specificity | Expressed in testicular cells. |
| Posttranslational modification | Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability (By similarity).Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters (By similarity).Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication (By similarity).Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MAP3K20 isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin (By similarity).Ubiquitinated.Lysine deamination at Lys-5 (H3K4all) to form allysine is mediated by LOXL2. Allysine formation by LOXL2 only takes place on H3K4me3 and results in gene repression (By similarity). |
| Celluar localization | Extracellular region or secreted;Nucleus; |
| UniPort | Q16695 |
Western blot analysis of extracts of HeLa cellline and H3 protein expressed in E.coli., usingH3K27me1antibody|Dot-blot analysis of all sorts of methylation peptides using H3K27me1 antibody|Immunofluorescence analysis of 293T cell using H3K27me1 antibody. Blue: DAPI for nuclear staining.
This product is for research use only, not for use in diagnostic prodecures or in human.
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