Rabbit Anti-CD8A Polyclonal Antibodyabs131203

abs131203-50ug

1-2 Weeks

201

abs131203-100ug

1-2 Weeks

301

Description

These cells are known as cytotoxic or suppressor T-cells and are responsible for interacting with targets that bear MHC class I molecules. The CD8 molecule is believed to play a crucial role in the T-cell mediated killing process. CD8 alpha chains bind specifically to the alpha-3 domains of class I MHC molecules, aiding in the identification and elimination of harmful cells.

Other names

Alpha polypeptide (p32), also known as CD8A antigen or CD8 antigen alpha polypeptide, is a crucial molecule involved in T-lymphocyte function. CD8A, also referred to as the Leu2 antigen, is a T-cell surface glycoprotein that acts as a co-receptor for the T-cell receptor (TCR). Its presence on the surface of T-cells aids in the recognition and binding of major histocompatibility complex class I (MHC-I) molecules displayed by antigen-presenting cells. This interaction enhances the T-cell's ability to respond to specific antigens and facilitate immune responses.
The CD8A molecule, encoded by the CD8A gene, is composed of an alpha chain that associates with a CD8 beta chain to form the CD8 heterodimer. The alpha chain is crucial for mediating the interaction with MHC-I molecules, while the beta chain provides structural support and stability to the CD8 complex.
The expression of CD8A is primarily restricted to cytotoxic T-cells and a subset of natural killer (NK) cells. It plays a vital role in T-cell development, activation, and differentiation. CD8A-positive T-cells, also known as CD8+ T-cells or cytotoxic T-cells, are responsible for targeting and eliminating infected or cancerous cells. They can recognize antigenic peptides presented by MHC-I molecules and subsequently induce cytotoxicity or produce various cytokines to regulate immune responses.
Alterations in CD8A expression or function can impact T-cell signaling and immune response. CD8A deficiencies or mutations may lead to impaired T-cell development and function, making individuals more susceptible to infections and immunological disorders.
In summary, CD8A is a key molecule involved in T-cell immunity, acting as a co-receptor for the TCR and facilitating T-cell activation and cytotoxicity. Its role in immune responses underscores its significance in understanding and potentially targeting immune-related diseases and conditions.

Source

Rabbit

Specificity

The presence of total CD8A can be detected by the CD8A Antibody, which is capable of identifying the endogenous levels. To maintain the integrity of the original text, rearranging the information can be done in the following manner: "Detection of total CD8A at endogenous levels is achievable with the CD8A Antibody."

Application

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A synthesized peptide derived from human CD8A.

Properties

1mg/ml

Purification

The SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography in order to purify the antiserum.

Clonality

Polyclonal Antibody

It is recommended to store at -20 °C for a year without subjecting it to repeated freeze/thaw cycles.

Storage buffer

This product contains Rabbit IgG in phosphate buffered saline (pH 7.4) with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C and is stable for 12 months from the date of receipt.

Target

Background

CD8A is an integral membrane glycoprotein crucial for immune responses. It serves multiple functions in fighting both external and internal threats. Specifically, in T-cells, CD8A acts as a coreceptor for MHC class I molecule:peptide complex. MHC class I peptides originate from cytosolic proteins, while class II peptides derive from extracellular proteins. CD8A interacts with both the T-cell receptor (TCR) and MHC class I proteins presented by antigen presenting cells (APCs). This interaction recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. Consequently, LCK phosphorylates various substrates, initiating intracellular signaling pathways that lead to lymphokine production, motility, adhesion, and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to detect and eliminate infected cells and tumor cells. In NK-cells, CD8A homodimers confer a survival mechanism by facilitating conjugation and lysis of multiple target cells. Furthermore, CD8A homodimer molecules support the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

Tissue specificity

CD8 on thymus-derived T-cells usually consists of a disulfide-linked alpha/CD8A and a beta/CD8B chain. Less frequently, CD8 can be expressed as a CD8A homodimer. A subset of natural killer cells, memory T-cells, intraepithelial lymphocytes, monocytes and dendritic cells expresses CD8A homo-dimers. Expressed at the cell surface of plasmacytoid dendritic cells upon herpes simplex virus-1 stimulation.

Celluar localization

Extracellular region or secreted;Plasma Membrane;

P01732