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Rabbit Anti-NFATc2IP Polyclonal Antibody#abs140482

Please note that the price mentioned above is for your reference only. For detailed pricing information, kindly get in touch with our sales representative, Vecent. Western blot analysis NFATc2IP using K562 whole cell lysates This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs140482-100ug	1-2 Weeks	301.0
abs140482-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is for your reference only. For detailed pricing information, kindly get in touch with our sales representative, Vecent.

Overview
catalog	abs140482
Other names	The 45 kDa NF-AT-interacting protein, also known as NF2IP_HUMAN or NIP45, is a cytoplasmic molecule that interacts with the nuclear factor of activated T-cells (NFAT). It is also referred to as ESC2 or NFATC2-interacting protein. This protein plays an important role in regulating the activity of NFAT, which is involved in the immune response and other cellular processes. Additionally, it is a calcineurin-dependent protein that interacts with the cytoplasmic form of NFAT. Another name for this protein is RAD60. Overall, the 45 kDa NF-AT-interacting protein is a crucial factor in modulating the function of NFAT and contributes to various cellular activities.
Source	Rabbit
Specificity	The total NFATc2IP can be effectively detected by the NFATc2IP Antibody, which is designed to target the endogenous levels of this protein. The presence of NFATc2IP can be accurately determined using this antibody, allowing for the analysis of various biological processes and pathways. By rearranging the original information, a new and distinct content has been generated.
Species Reactivity	Human
Antigen	NFATc2IP
Application	For Western Blot analysis, use a dilution of 1:1000. For Immunofluorescence/Immunocytochemistry, use a dilution range of 1:100 to 1:500. For ELISA using peptides, use a dilution range of 1:20000 to 1:40000. It is important to note the specific dilution ranges for each application in order to achieve accurate and reliable results.
Immunogen	A synthesized peptide derived from human NFATc2IP.
MW	48kDa
Properties
Concentration	1mg/ml
purification	Purification of the antiserum involved the use of SulfoLink™ Coupling Resin in peptide affinity chromatography. A highly similar content can be generated by rearranging the wording as follows: The SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography in order to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure proper storage, keep the product at a temperature of -20 °C for one year. It is important to avoid subjecting it to repeated freeze/thaw cycles.
Storage buffer	The Rabbit IgG solution comes in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide and 50% glycerol. Storage at -20°C is recommended and the solution will be stable for up to 12 months from the date of receipt. This information may be helpful for those who are considering purchasing or using this product.
Target
Background	In T-helper 2 (Th2) cells, regulates the magnitude of NFAT-driven transcription of a specific subset of cytokine genes, including IL3, IL4, IL5 and IL13, but not IL2. Recruits PRMT1 to the IL4 promoter; this leads to enhancement of histone H4 'Arg-3'-methylation and facilitates subsequent histone acetylation at the IL4 locus, thus promotes robust cytokine expression (By similarity). Down-regulates formation of poly-SUMO chains by UBE2I/UBC9 (By similarity).
Posttranslational modification	Methylation at the N-terminus by PRMT1 modulates interaction with the NFAT complex and results in augmented cytokine production.
Celluar localization	Nucleus;
UniPort	Q8NCF5