Rabbit Anti-BAZ2A Polyclonal Antibody#abs140937
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Description
| Catalog-specification | Delivery time | USD price |
abs140937-100ug | 1-2 Weeks | 301.0 |
abs140937-50ug | 1-2 Weeks | 201.0 |
Please be informed that the mentioned price is provided for your reference only. For detailed pricing information, we kindly request you to get in touch with our sales representative, Vecent. Please note that the generated content should be different from the original text and should not be created in the form of a conversation like ChapGPT.
| Overview | |
catalog | abs140937 |
| Source | Rabbit |
| Specificity | The total BAZ2A levels in cells can be detected using the BAZ2A Antibody, which specifically binds to the endogenous BAZ2A protein. |
| Species Reactivity | Human |
| Predictive reaction species | Pig;Rabbit;Sheep;Dog;Horse;Bovine; |
| Antigen | BAZ2A |
| Application | The dilutions for Western blot (WB) range from 1:500 to 1:1000, while for immunofluorescence (IF)/immunocytochemistry (ICC) they range from 1:100 to 1:500. In the case of ELISA using peptide antigens, the recommended dilutions fall between 1:20000 and 1:40000. It is important to note that these dilutions are crucial for obtaining accurate and reliable results in these experimental techniques. |
| Immunogen | A synthesized peptide. |
| MW | 210 KD |
| Properties | |
Concentration | 1mg/ml |
| purification | Peptide affinity chromatography was employed for the purification of the antiserum using SulfoLink™ Coupling Resin. The process involved binding the target peptides to the resin and then washing off non-specifically bound proteins, resulting in a highly purified antiserum. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Ensure that the storage temperature is maintained at -20 °C for a duration of one year. It is important to avoid subjecting the product to multiple freeze/thaw cycles during this time. |
| Storage buffer | This product contains Rabbit IgG dissolved in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is advised to store this product at -20 °C, as it remains stable for up to 12 months from the date of receipt. |
Target | |
Background | The NoRC complex is essential for silencing a portion of rDNA, and this is achieved by recruiting histone-modifying enzymes and DNA methyltransferases. A key component of this complex is responsible for targeting these enzymes and facilitating the repression of RNA polymerase I transcription. It accomplishes this by being recruited to the rDNA region through its interaction with TTF1 and its ability to recognize and bind acetylated histone H4 on Lys-16 (H4K16ac), which results in the deacetylation of other lysine residues on H4, but not on H4K16ac. This component specifically binds to pRNAs, which are complementary to the rDNA promoter sequence and are 150-250 nucleotides in length. The formation of heterochromatin and silencing of rDNA is dependent on the binding of pRNAs to this component. |
| Tissue specificity | Expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. |
| Posttranslational modification | Acetylation at Lys-680 by KAT8/MOF promotes its dissociation from pRNA, affecting heterochromatin formation, nucleosome positioning and rDNA silencing. Deacetylation by SIRT1 in late S phase enhances pRNA-binding, allowing de novo DNA methylation and heterochromatin formation. Acetylation is high during S phase and declines to background levels in late S phase when the silent copies of rRNA genes are replicated (By similarity).Ubiquitinated. Deubiquitinated by USP21 leading to its stabilization. |
| Celluar localization | Cytosol;Nucleus; |
| UniPort | Q9UIF9 |
This product is for research use only, not for use in diagnostic prodecures or in human.
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