Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-ARFGEF2 Polyclonal Antibody#abs140936

Please note that the price mentioned above is only for reference purposes. For detailed pricing information, we kindly request you to contact our seller Vecent. Western blot analysis of extracts from A549 cells, using ARFGEF2 antibody. This product is for research use only, not for use in...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs140936-100ug	1-2 Weeks	301.0
abs140936-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for reference purposes. For detailed pricing information, we kindly request you to contact our seller Vecent.

Overview
catalog	abs140936
Other names	ARF guanine nucleotide exchange factor 2, also known as BIG2, is an enzyme that plays a critical role in the intracellular trafficking of proteins. It is a member of the ADP ribosylation factor guanine nucleotide exchange factor family, which is involved in the regulation of vesicle transport and organelle structure. BIG2 is inhibited by brefeldin A, a fungal metabolite that disrupts the Golgi apparatus and inhibits protein secretion. The gene for BIG2, known as ARFGEF2, is located on chromosome 3 and encodes a 1834 amino acid protein. It is expressed in a variety of tissues, including the brain, liver, and pancreas. Mutations in ARFGEF2 have been associated with a range of diseases, including neurological disorders, developmental delay, and cancer. Understanding the function of BIG2 may lead to new therapies for these conditions.
Source	Rabbit
Specificity	The ARFGEF2 Antibody is capable of identifying the overall levels of endogenous ARFGEF2 present in the system. To produce similar content, the information provided can be rephrased in a different manner while ensuring that the original text is not altered.
Species Reactivity	Human;Mouse;Rat
Antigen	ARFGEF2
Application	The dilution factors for Western Blotting (WB) range from 1:500 to 1:1000, while for Immunofluorescence (IF) or Immunocytochemistry (ICC), the dilution ranges from 1:100 to 1:500. For ELISA using peptide antigens, a dilution factor of 1:20000 to 1:40000 is recommended. It is important to maintain the original information and context while rearranging the content.
Immunogen	A synthesized peptide.
MW	210 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was subjected to peptide affinity chromatography in order to purify it.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage conditions, it is recommended to keep the product at a temperature of -20°C for up to one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this can have a negative impact on its quality. Ensuring proper storage conditions can help to maintain the stability and efficacy of the product over an extended period of time.
Storage buffer	This product contains Rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4, 150mM of NaCl, 0.02% of sodium azide, and 50% glycerol. For optimum storage, please keep this solution at a temperature of -20 °C. This product will remain stable for up to 12 months from the date of receipt.
Target
Background	Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extent on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways.
Tissue specificity	Expressed in placenta, lung, heart, brain, kidney and pancreas.
Posttranslational modification	In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1.
Celluar localization	Cytoskeleton;Cytosol;Endosome;Golgi apparatus;
UniPort	Q9Y6D5